Battelle Memorial Institute

Craig Bartling

Anthony David Duong

Esley M Heizer

Mark E Hester

Lena Furci

Carrie Howland

Trevor Petrel

Ramanathan S Lalgudi

Cherry Gupta

Rachel R Spurbeck

Miguel D Pedrozo

Nickolas R Andrioff

Katarzyna Kucharzyk

Jane P Fife

James Ha

Lena M Furci

The present disclosure is directed to compositions and methods that use the principles of DNA origami to package and archive data stored in multiple indexed DNA oligonucleotides. These structures allow for selective physical data access and retrieval from a molecular pool of DNA origami (DNAO) nanostructures comprising the data bearing oligonucleotides.

Use of DNA origami nanostructures for molecular information based data storage systems

An agricultural composition comprises living microorganisms encapsulated in polymer gel microbeads which are applied onto seeds. In another embodiment, an agricultural composition comprises living microorganisms encapsulated in polymer gel macroheads for planting with seeds in soil. In another embodiment, an agricultural composition comprises living microorganisms immobilized in a polymer gel matrix that is coated on a seed.

Encapsulation for microbial seed treatment stabilization

Illustrative embodiments of systems and methods for the identification of traits associated with DNA samples using epigenetic-based patterns detected via massively parallel sequencing (MPS) are disclosed. Illustrative embodiments may involve digesting a DNA sample with a methylation-dependent endonuclease, amplifying loci of the digested DNA sample (including a positive control locus that does not contain a restriction site for the methylation-dependent endonuclease) using a multiplex PCR to produce amplicons, sequencing the amplicons using an MPS instrument to generate sequence reads, determining a sequence count for each of the loci by comparing each of the sequence reads to reference sequences, normalizing the sequence count for each of the loci to the sequence count of the positive control locus, and identifying a trait associated with the DNA sample by applying a classification algorithm to the normalized sequence counts.

Identification of traits associated with DNA samples using epigenetic-based patterns detected via massively parallel sequencing

Processes and apparatuses for producing biologically-active, protein-rich microparticles under ambient conditions are disclosed. A protein solution is atomized and collected in a dehydration solvent that is being mixed. The resulting protein microparticles retain high specific activity without the need for large amounts of stabilizing excipients.

Processes for producing protein microparticles

Growing community of inventors

Lewis Center, OH, United States of America

Craig Bartling