Other

Rhein Biotech

Cornelius P Hollenberg

Alexander Wm Strasser

Feodor B Martens

Jurgen Dohmen

Zbigniew Janowicz

The invention relates to DNA-molecules comprising DNA-sequences encoding control regions and the structural gene for a protein having formate dehydrogenase (FMDH) activity. Said DNA-molecules may be combined with DNA-sequences encoding foreign genes so as to bring these genes under the stringent control of the regulation of the FMDH regulatory sequences and/or may be combined to DNA-sequences coding for secretory signals. The invention further relates to recombinant vectors containing said DNA-molecules and micro-organisms containing said vectors or DNA-molecules. Furthermore, the invention relates to a process for producing a useful substance by producing this substance by culturing said micro-organisms and recovering the substance.

DNA-molecules coding for FMDH control regions and structural gene for a

This invention provides a method for producing amylolytic enzymes by culturing a microorganism, having received as a result of recombinant DNA technology DNA sequences from a donor yeast comprising the coding sequences for the amylolytic enzymes wherein the host microorganism is capable of expressing said amylolytic enzymes. Furthermore, this invention provides microorganisms genetically engineered as to being able to produce and express the amylolytic enzymes, a vector containing the DNA sequences, coding for the amylolytic enzymes and the respective DNA sequences. The said host microorganisms are useful in the production of biomass and many fermentation processes, preferably in the production of special beers.

Fermentation processes using amylolytic enzyme producing microorganisms

The cloning of Schwanniomyces glucoamylase and alpha-amylase genes is taught. The genes are expressed in recombinant host cells.

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Dusseldorf, Germany

Cornelius P Hollenberg