Genencor International, Inc.

Cornelis P Broekhuizen

Wilhelmus J Quax

Richard Kerkman

Jan Metske Van Der Laan

Onno Misset

Erik Jan Baas

Franciscus Josephus Cornelius Van Gastel

Leonardus Johannes Sofie Marie Mulleners

Heterologous protein secretion from Gram-positive bacteria, in particular from Bacilli has, with few exceptions, met with little success. Incompatibility of the heterologous proteins with the protein secretion machinery of the host is the main cause of this effect. This limiting factor for the production of heterologous proteins in commercially significant concentrations from is removed by overexpressing the protein FtsY or FtsY protein in combination with overexpression of other members of the bacterial signal recognition particle. Said gene(s) is(are) overexpressed in Bacillus host cells expressing a heterologous protein which then shows an increased amount of the heterologous protein secreted in the surrounding medium.

Secretion factors for gram-positive micoorganisms, genes encoding them and methods of using it

Secretion factors for gram-positive microorganisms genes encoding them and methods of using it

High alkaline serine protease

Growing community of inventors

Rijswijk, Netherlands

Cornelis P Broekhuizen