Rijk Zwaan Zaadteelt En Zaadhandel B.v.

Mogen International Nv

Syngenta Mogen B.v.

Ses Europe N.v./s.a.

Cornelis Maria Petrus Van Dun

Johannes Wilhelmus Schut

Robert Helene Ghislain Dirks

Jacobus Petrus Cornelis De Wit

Pieter Martijn Eggink

Joyce Sylvia Velterop

Kornelius Reinink

Beatrice Ingrid Lindhout

Willem Van Vliet

Oscar Johannes Maria Goddijn

Dörthe Bettina Dräger

Silvester De Nooijer

Johannis Hendrik De Rooij

Raoul Jacobus Johannes Maria Frijters

Cornelis Haaring

Lena Johanna Huijbregts-Doorduin

Dorthe Bettina Drager

Jacob Pieter Willem Haanstra

Hubert Guilley

Sara Movahedi

Jasper De Joode

Robert Hélène Ghislain Dirks

Magdalena Barbara Lastdrager

Petrus Lambertus Josephus Egelmeers

Klaus-Peter Krause

Joannes Wilhelmus Schut

Johannes Willem Kloet

Petrus Lambertus J Egelmeers

Japser De Joode

Kenneth Richards

Eric Cornelis Josephus Bal

Gérard Jonard

Hendrik Tigelaar

Doerthe Bettina Draeger

Dörthe Bettina DRÄGER

Pieter Marijn Eggink

Johan Schut

Velterop Joyce Sylvia Sylvia

Kornelis Reinink

The present invention relates a plant which may comprise a modified F5H gene homolog, wherein said gene homolog may comprise a modification as compared to its corresponding wild type F5H gene homolog, wherein the presence of the modified F5H gene homolog in the plant leads to a reduction of wound-induced surface discoloration in comparison to a plant not comprising the modified F5H gene homolog. The invention also relates to a modified F5H gene homolog that leads to the reduced wound-induced surface discoloration. The invention further relates to use of the gene in breeding and producing plants that show reduced wound-induced surface discoloration.

Plants showing a reduced wound-induced surface discoloration

The invention relates to a modified PIN4 gene, the wild type of which is as identified in SEQ ID No. 1, encoding the protein of SEQ ID No. 5, or the wild type of which encodes a protein that has a sequence similarity of at least 80% to SEQ ID No. 5, which modified PIN4 gene encodes a protein that comprises an amino acid change as a result of the modification, and which modified protein is capable of inducing parthenocarpic fruit set when present in a plant. The invention also relates to plants and fruits that carry the gene. Such plants are capable of parthenocarpic fruit set and the fruits do not contain seeds. The invention further relates to use of the gene in breeding and producing plants capable of parthenocarpic fruit set.

Modified gene resulting in parthenocarpic fruit set

The present invention relates to plants of the Compositae family exhibiting a reversible genic male sterility trait, characterised in that the genic male sterility may be caused by a reduction or complete absence of endogenous jasmonic acid production, resulting from interference with one or more target genes involved in endogenous jasmonic acid production, selected from the group consisting of lipoxygenase, allene oxide synthase, allene oxide cyclase and 12-oxo-phytodienoic acid-10,11-reductase, or their functional homologues.

Reversible genic male sterility in compositae

The present invention relates to modified RDR1 gene capable of conferring virus resistance to a plant and/or increasing virus resistance in a plant, which modification results in enhanced expression of the RDR1 gene, and wherein the modification is selected from a modification that increases the mRNA level of the RDR1 gene; a modification that increases the level of the RDR1 protein; and/or a modification that increases the activity of the RDR1 protein, as compared to a non-modified wild-type RDR1 gene. The modification may comprise a modification upstream of the coding sequence of the RDR1 gene, such as a modification of a regulatory element, preferably of a cis-acting regulatory element. The modified regulatory element is for example selected from a transcription factor binding site for a transcriptional repressor, the modification of which leads to reduction or absence of transcriptional repression; and/or a transcription factor binding site for a transcriptional activator, the modification of which leads to induction or enhancement of transcription; and/or a microRNA binding site, the modification of which leads to reduction or absence of gene repression; and/or a small RNA sequence, the modification of which leads to reduction or absence of gene repression.

Modified gene conferring virus resistance

The present invention relates to a non-destructive method for analyzing maternal DNA of a seed. In this method the DNA may be dislodged from the seed coat surface and may be used to collect information on the genome of the maternal parent of the seed. Also, the present invention provides a high throughput DNA analysis system for large plant populations.

Method for analyzing maternal DNA in large plant populations

The present invention relates to plants of the Compositae family exhibiting a reversible genic male sterility trait, characterized in that the genic male sterility is caused by a reduction or complete absence of endogenous jasmonic acid production, resulting from interference with one or more target genes involved in endogenous jasmonic acid production, selected from the group consisting of lipoxygenase, allene oxide synthase, allene oxide cyclase and 12-oxo-phytodienoic acid-10, 11-reductase, or their functional homologues.

The invention relates to a method for the production of a set of seeds which are genetically identical to the male gametes from which they arise, which may comprise placing a limited number of paternal gametes that have the form of tetrads or dyads on the stigma of a flower to fertilize maternal egg cells to obtain a number of zygotes; and inducing the loss of maternal chromosomes from the zygotes to obtain a seed set containing a limited number of seeds in which the maternal chromosomes are absent. In a preferred embodiment the father plant exhibits suppression of chromosome recombination or second division restitution (SDR) during meiosis.

Quartet breeding

The invention relates to a tomato plant, wherein the fruits of which have an improved shelf-life as compared to the fruits of a wild type tomato plant, wherein the genetic determinant causative of the improved shelf life trait is a mutation in the hp2 gene. The increased shelf-life may comprise a fruit that shows normal ripening having a fruit firmness at red ripe harvest that is increased by at least 31%, preferably by at least 42%, more preferably by at least 52%, even more preferably by at least 60%, most preferably by at least 70% as compared to a fruit having similar genetic background that lacks the trait of the invention.

Tomato with improved shelf-life

A method for efficiently producing homozygous organisms from a heterozygous non-human starting organism, comprising providing of a heterozygous starting organism; allowing the starting organism to produce haploid cells; creating homozygous organisms from the haploid cells thus obtained; and selecting the organisms having the desired set of chromosomes; wherein during production of the haploid cells no recombination occurs in order to obtain a limited number of genetically different haploid cells. Recombination can also be prevented or suppressed.

Reverse breeding

The present invention relates to plants of the Compositae family exhibiting a reversible genic male sterility trait, characterized in that the genic male sterility may be caused by a reduction or complete absence of endogenous jasmonic acid production, resulting from interference with one or more target genes involved in endogenous jasmonic acid production, selected from the group consisting of lipoxygenase, allene oxide synthase, allene oxide cyclase and 12-oxo-phytodienoic acid-10,11-reductase, or their functional homologs.

The invention relates to a tomato plant the fruits of which have an improved shelf-life as compared to the fruits of a wild type tomato plant, obtainable by introgressing the increased shelf life trait from the mutant LePQ58 (deposit accession number NCIMB 41531) into a tomato plant with a normal shelf-life. The increased shelf-life may comprise a fruit that shows normal ripening having a fruit firmness at red ripe harvest that is increased by at least 31%, preferably by at least 42%, more preferably by at least 52%, even more preferably by at least 60%, most preferably by at least 70% as compared to a fruit having similar genetic background that lacks the trait of the invention.

Provided is a method for producing a homozygous non-human organism from a heterozygous non-human organism, which homozygous organism can be crossed to obtain a hybrid, comprising providing a heterozygous starting organism; allowing the organism to produce SDR-0 cells through meiosis, which cells originate from second division restitution; regenerating SDR-0 organisms from the SDR-0 cells; and producing the homozygous organism from the SDR-0 organisms thus obtained. Further provided is a method for producing a hybrid, comprising crossing a first homozygous organism that is produced according to the above method with a second homozygous organism. Also provided is a homozygous non-human organism and hybrid non-human organisms obtainable by these methods. In a preferred embodiment, the organisms are plants.

Near reverse breeding

The invention relates to a method for testing a plant or plant part for exhibiting a reduced or absent wound-induced surface discoloration as compared to a control plant or plant part, which method comprises providing a plant or plant part that is obtained from a plant population that shows genetic variation, in particular a gene bank; optionally creating a wound surface on the plant or plant part; incubating the plant or plant part or the wound surfaces created thereon to allow for discoloration to occur therein or thereon; observing the discoloration in or on the plants or plant parts; comparing the observed discoloration with the discoloration that is observed in the control plant or plant part to assess whether the plant or plant part shows no discoloration or a discoloration that is reduced as compared to the control plant or plant part; and identifying a plant or plant part that shows no discoloration or a discoloration that is reduced as compared to the control plant or plant part as a plant that harbors the reduced wound-induced surface discoloration trait.

Method for identifying plant material that shows a reduced discolouration, plants thus identified and use of the identified plants for the production of commercial crop plants showing a reduced discolouration

Growing community of inventors

Roosendaal, Netherlands

Cornelis Maria Petrus Van Dun