Academisch Ziekenhuis Leiden (h.o.d.n. Lumc)

Rijksuniversiteit Leiden

Seed Capital Investments (sci) B.v.

Academish Ziekenhuis Leiden H.o.d.n. Lumc

Cornelis Johannes Melief

Jan Wouter Drijfhout

Gemma G Kenter

Sjoerd Henricus Van Der Burg

Jan Kessler

Marieke Griffioen

Wybe Martin Kast

The present invention relates to novel CD4+ and CD8+ T cell epitopes that are specific for HPV-specific E6 and E7 oncoproteins, to peptides comprising these novel T cell epitopes, and to (vaccine) compositions comprising these peptides for use in methods for the prevention and/or treatment of HPV related diseases. Preferred epitopes are recognized by a T cell that infiltrates a cervical neoplastic lesion or by a T cell from a draining lymph node, and are presented by an HLA-DQ or HLA-DP molecule, or an HLA-B.

HPV epitopes targeted by T cells infiltrating cervical malignancies for use in vaccines

The invention relates to a peptide having a length of no more than 100 amino acids and comprising at least 19 contiguous amino acids from the amino acid sequence of the human PRAME protein, wherein the peptide comprises at least one HLA class II epitope and at least one HLA class I epitope from the amino acid sequence of the human PRAME protein and to its use as such or in a composition as a medicament for the treatment and/or prevention of cancer.

PRAME derived peptides and immunogenic compositions comprising these

Induction of an antigen-specific T-lymphocyte response in a T-lymphocyte culture, e.g. a primary cytotoxic T-lymphocyte (CTL) response, by loading antigen-presenting vehicles which carry empty MHC molecules with an antigen-derived T-cell-immunogenic MHC-binding peptide, culturing T-lymphocytes in the presence of the peptide-loaded antigen-presenting vehicles under specific T-lymphocyte response-inducing conditions. Optionally, an antigen-specific T-lymphocyte is isolated from the resulting culture and cultured. The process can be used for preparing CTL which are specific for viral or other foreign antigens, or CTL which are specific for autologous peptides. The process can also be used for the identification of peptides that are capable of binding to MHC and inducing a T cell response.

Induction of antigen specific T-lymphocyte responses by stimulation with

Growing community of inventors

Haarlem, Netherlands

Cornelis Johannes Melief