Dyadic International (usa), Inc.

Cornelia Maria Johanna Van Zeijl

Peter Jan Punt

Mark Aaron Emalfarb

Richard Paul Burlingame

Arkady Panteleimonovich Sinitsyn

Philip Terry Olson

Christine Marie Pynnonen

Jean Christophe Bousson

Martine Parriche

A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus, more particularly ofand mutants or derivatives thereof. It also covers transformants containingcoding sequences, as well expression-regulating sequences ofgenes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.

Transformation system in the field of filamentous fungal hosts

The invention pertains to novel proteins corresponding toglycosyl hydrolases of families 7 and 10, exhibiting a minimum aminoacid identity of 70 and 75%, respectively, with the amino acid sequence of SEQ ID No's 2 and 4, and to a protein corresponding to aglyceraldehyde phosphate dehydrogenase, exhibiting at least 86% amino acid identity with the partial amino acid sequence of SEQ ID No. 6. The invention further relates to nucleic acid sequences encoding these proteins, and especially to promoter sequences regulating the expression of the corresponding genes. The preferred host for expressing these genes is a fungus, especially astrain.

Expression-regulating sequences and expression products in the field of filamentous fungi

Growing community of inventors

Vleuten-de-Meern, Netherlands

Cornelia Maria Johanna Van Zeijl