Boehringer Mannheim Gmbh

Cornelia Kruse-Muller

Joachim Ziegenhorn

Joachim Siedel

Christoph Kessler

Rudolf Seibl

Sibylle Berner

Rudiger Ruger

Method for the specific detection of nucleic acids in a sample by reaction of the sample with one or several labelled nucleotide triphosphates and one or several enzymes which catalyse the production of a labelled nucleic acid B which contains this nucleotide, non-thermally denaturation, reacting the sample with a nucleic acid probe C which is sufficiently complementary to nucleic acid B and which contains at least one immobilizable group, if desired, contacting the nucleic acid hybrid D formed with a solid phase which recognizes and binds the immobilizable group, removing the liquid from the solid phase and determining the label on the solid phase as a measure for the presence of the nucleic acid.

Method for the sensitive detection of nucleic acids

The present invention provides a process for the determination of total bilirubin in samples of body fluids. The method comprises the incubation of a sample of body fluid with both subtilisin which completely liberates bilirubin from its albumin and with bilirubin oxidase as a measurement of total bilirubin content in said body fluid sample.

Process and reagent for the determination of total bilirubin

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Tutzing, Germany

Cornelia Kruse-Muller