Other

University Of Zürich

Cornel Fraefel

Mathias Ackermann

Xandra O Breakefield

E Antonio Chiocca

Mark Suter

Gosse Jan Adema

Yoshinaga Saeki

Bruna De Andrade Pereira

Ken Shortman

Kurt Tobler

Christiane Dresch

The invention relates to transgene expression constructs&#x2014;particularly self inactivating lentiviral vectors&#x2014;comprising a dendritic cell specific promoter controlling the expression of autoantigen proteins, namely myelin basic protein, proteolipid protein and myelin oligodendrocyte glycoprotein, for use in the therapy of multiple sclerosis.

APC-mediated tolerance induction for therapy of multiple sclerosis

The present invention relates to an enhanced and simplified herpes virus amplicon packaging system. The packaging system comprises a herpes virus amplicon vector and a packaging vector. In one embodiment, the packaging vector comprises a bacterial artificial chromosome (BAC) containing the HSV-1 genome. The packaging vector contains an intact pac site but is otherwise rendered packaging defective. The packaging vector can be rendered packaging defective by inserting nucleotides into the pac site, or by otherwise interfering with the capsid's ability to close, for example, by increasing the size of the DNA fragment upon which the herpes virus genome is cloned. This system can be used to package a wide range of nucleotide sequences (e.g., a therapeutic or antigenic gene) into an empty herpes virus particle taking advantage of the large transgene capacity of herpes viruses. This system can also be used as a vaccine to induce protective immunity against HSV-1, or other complex pathogens.

Enhanced packaging of herpes virus amplicons and generation of recombinant virus vectors

Growing community of inventors

Trüllikon, Switzerland

Cornel Fraefel