Basf Aktiengesellschaft

Basf Se Corporation

Evonik Degussa Gmbh

Paik Kwang Industrial Co., Ltd.

Basf Aktiencesellschaft

Corinna Klopprogge

Oskar Zelder

Hartwig Schröder

Burkhard Kröger

Stefan Haefner

Gregor Haberhauer

Markus Pompejus

Andrea Herold

Hartwig Schroder

Stefan Häfner

R Rogers Yocum

Mark K Williams

Christoph Wittmann

Thomas A Patterson

Weol Kyu Jeong

Elmar Heinzle

Wolfgang Grabarse

Janice G Pero

Uwe Sauer

Heung-Shick Lee

Patrick Kiefer

Claudia Isabella Graef

Uwe Ruffer

Jörg Mampel

Theron Herman

Jens Kroemer

Anja Knietsch

Hartwig SCHRÖDER

Mark Williams

The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant microorganism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.

Process for the production of gamma-aminobutyric acid

Process for the production of cadaverine by constructing a recombinant microorganism which has a deregulated lysine decarboxylase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diaminopimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism.

Process for the production of cadaverine

The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.

PEF-TS expression units

Pgro expression units

The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a &#x394;metF organism or a &#x394;metE AmetH organism, for example, mutants ofor, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/MetE and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A &#x394;metF &#x394;metB strain ofcan use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

Use of dimethyl disulfide for methionine production in microoraganisms

The present invention relates to nucleotide sequences encoding enzymatically active cobalamin-methionine synthase and functional fragments thereof that are modified in comparison to the respective wild-type enzyme such that said enzymes show reduced product inhibition by methionine. The present invention also relates to polypeptides being encoded by such nucleotide sequences and host cells comprising such nucleotide sequences. Furthermore, the present invention relates to methods for producing methionine in host organisms by making use of such nucleotide sequences.

Nucleic acid encoding a cobalamin-dependent methionine synthase polypeptide

The present invention relates to microorganisms and methods for producing methionine by reactivation of the MetH enzyme.

Microorganisms with a reactivation system for cob(I)alamin-dependent methionine synthase

The present invention relates to microorganisms and processes for the efficient preparation of L-amino acids such as L-methionine. In particular, the present invention relates to microorganisms and processes in which the formation and/or accumulation of homolanthionine in the methionine pathway is reduced and/or prevented.

Microorganism and process for the preparation of L-methionine

Psod expression units

The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g.,by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine inby way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

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P EF-TU expression units

The present invention relates to multiple promoters and to expression units comprising them; to the use thereof for regulating transcription and expression of genes; to expression cassettes which comprise multiple promoters or expression units of this kind; to vectors which comprise such expression cassettes; to genetically modified microorganisms which comprise vectors and/or expression units of this kind; and to processes for preparing biosynthetic products by culturing said genetically modified microorganisms.

Multiple promoters and the use thereof for gene expression

The invention relates to methods for the production of sulfur-containing fine chemicals, in particular L-methionine, by fermentation using bacteria in which a nucleotide sequence encoding an S-adenosylmethionine synthase (metK) gene is expressed.

Method for the production of sulpher-containing fine chemicals by fermentation

The present invention relates to mutated nucleic acids and proteins of the metabolic pathway of fine chemicals, to processes for preparing genetically modified producer organisms, to processes for preparing fine chemicals by culturing genetically modified organisms, and to genetically modified organisms themselves.

Gene variants coding for proteins from the metabolic pathway of fine chemicals

Growing community of inventors

Mannheim, Germany

Corinna Klopprogge