Abengoa Bioenergia Nuevas Tecnologias, S.a.

Abengoa Bioenergia Nuevas Technologias, S.a.

Consolación Álvarez Núñez

Antonio Javier Moreno Pérez

Laura Sánchez Zamorano

Dolores Pérez Gómez

Sandra Gavaldá Martín

Noelia Valbuena Crespo

Bruno Díez García

Laura Viñas De La Cruz

Ana Isabel Platero Gómez

María De Los Ángeles Bermúdez Alcántara

Javier Rocha Martín

Francisco Reyes Sosa

Laura Ledesma García

Lucía Martín Pérez

Juan Luis Ramos Martín

Ricardo Arjona Antolín

María De Los Angeles Bermúdez Alcántara

Lucia Martín Pérez

Laura Benítez Casanova

Macarena López Morales

Ana Gomez Rodriguez

Jorge Gil Martinez

Rafael Duenas Sanchez

Ana Maria Munoz Gonzalez

Pablo Gutierrez Gomez

The invention relates to a host cell, preferably acell, which presents a lower expression and/or secretion of non-contributory cellulolytic enzymes, preferably where the non-contributory cellulolytic enzyme is endoglucanase 6 comprising SEQ ID NO: 2, thereby promoting the presence of contributory cellulolytic enzymes in the enzymatic cocktail synthesised by said host cell. The invention also relates to the use of said host cells and the enzymatic cocktails synthesised by said host cells for the production of fermentable sugars of biomass and a method for producing bioproducts, preferably bioethanol, comprising the use of said host cell or the composition according to the invention.

host cell having improved cellulolytic activity and enzymatic compounds produced with same

The invention refers to methods and compositions for stabilizing and increasing the activity of enzymatic mixtures comprising GH61 (PMO or polysaccharide monooxigenase) polypeptides used for the degradation of cellulosic material during the saccharification step of biofuel production processes. This improvement is achieved by the addition of a nickel cation to said enzymatic mixtures before and/or during the saccharification step. Thus, the invention provides compositions comprising PMOs, cellulolytic enzymes and a nickel cation, as well as methods for preparing said compositions and methods for producing fermentable sugars and bioproducts, preferably bioethanol, from cellulosic biomass in which said compositions are used.

Cellulolytic compositions comprising monooxygenase polysaccharide enzymes with improved activity

The present invention relates to variants of cellobiohydrolase, preferably Cbh1, which have greater cellobiohydrolase activity. The invention also relates to a genetic construct, a host cell and to an enzyme composition comprising said variants. The invention further relates to a procedure for producing fermentable sugar and a procedure for producing a bioproduct, such as bioethanol, from cellulose material with the cellobiohydrolase variants, the host cell or the enzyme composition comprising said variants.

Variants of cellobiohydrolase 1

The present invention relates to ahost cell which expresses a recombinant enzymes fromwith beta-xylosidase activity. The invention also refers to an enzymatic composition comprising the host cell of the invention and/or the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention. The invention further relates to the use of the host cell of the invention, the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention or the composition of the invention for the degradation of biomass and to a method of producing bioproducts, preferably bioethanol, which comprises the use of the host cell of the invention, the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention or the composition of the invention.

Expression of recombinant beta-xylosidase enzymes

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Seville, Spain

Consolación Álvarez Núñez