The Charles Stark Draper Laboratory, Inc.

Connor McBrine

Jason Wyatt Holder

Miles Rogers

Georgiana Kourepenos

Parker Dow

Nicole Billings

Kirsty A McFarland

Sarah Gruszka

Sarah Gruska

Lucas Tilley

The present disclosure provides methods of generating recombinant bacteriophage genomes via semi-synthesis. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a bacteriophage genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.

Bacteriophage engineering via semi-synthesis

The present disclosure provides methods of generating recombinant bacteriophage genomes. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a linear bacteriophage DNA genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.

Phage engineering: protection by circularized intermediate

The present disclosure provides compositions including recombinant B11 bacteriophages, methods for making the same, and uses thereof. The recombinant B11 bacteriophages disclosed herein are useful for the identification and/or antibiotic susceptibility profiling of specific bacterial strains/species present in a sample.

Recombinant B11 bacteriophages and uses thereof

The present disclosure provides methods and kits for generating recombinant bacteriophage genomes.

Bacteriophage engineering methods

The present disclosure provides methods and kits for generating recombinant bacteriophage genomes. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a bacteriophage DNA genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.

Bacteriophage recombination followed by blockage of non-recombinant bacteriophage replication

The present disclosure provides compositions including recombinant K1-5 bacteriophages, methods for making the same, and uses thereof. The recombinant K1-5 bacteriophages disclosed herein are useful for the identification and/or antibiotic susceptibility profiling of specific bacterial strains/species present in a sample.

Recombinant K1-5 bacteriophages and uses thereof

The present disclosure provides compositions including recombinant K2 bacteriophages, methods for making the same, and uses thereof. The recombinant K2 bacteriophages disclosed herein are useful for the identification and/or antibiotic susceptibility profiling of specific bacterial strains/species present in a sample.

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Somerville, MA, United States of America

Connor McBrine