Merck Sharp + Dohme Corp.

Schering Corporation

Collette Marie Cutler

Brittany C Paporello

Steven J Blaisdell

Alexandre Ambrogelly

Susan V Cannon-Carlson

Gary J Vellekamp

Marcio Voloch

Kimberly Margaret Louise May

Brittany Charlotte Larkin

Provided are methods of producing mono- and di-pegylated IL-10.

Mono- and di-PEG IL-10 production; and uses

Mono- and di-peg IL-10 production; and uses

Provided are methods of producing a mixture of mono- and di-pegylated IL-10.

The present invention includes a method for reducing the appearance of false positive bands in SDS-PAGE analysis of proteolytic digestion, of a sample that comprises an immunoglobulin polypeptide, at pH 8.8.

Method for reducing the appearance of false positive bands in SDS-page analysis of proteolytic digestion of a sample

Methods for purifying adenoviruses from a contaminated sample using a hydroxyapatite medium are provided. Sodium chloride concentrations present in buffers used throughout the method are at least 150 mM to prevent the adenovirus from irreversibly binding to the hydroxyapatite. Levels of contaminants and empty adenovirus capsids from samples purified by conventional purification techniques are further reduced to provide a highly pure adenovirus preparation.

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Bloomingdale, NJ, United States of America

Collette Marie Cutler