The University of British Columbia

University of California

Colin Walsh

Andre Wild

Timothy Leaver

Euan Ramsay

Robert James Taylor

Kevin Ou

Aysha Ansari

Pieter Rutter Cullis

Francis C Szoka, Jr

Nathan Maurice Belliveau

Anitha Thomas

R James Taylor

Vincent Venditto

Juliane Nguyen

Gesine Heuck

Emily Perttu

Euan Ramsey

The present disclosure is directed towards improved systems and methods for large-scale production of nanoparticles used for delivery of therapeutic material. The apparatus can be used to manufacture a wide array of nanoparticles containing therapeutic material including, but not limited to, lipid nanoparticles and polymer nanoparticles. In certain embodiments, continuous flow operation and parallelization of microfluidic mixers contribute to increased nanoparticle production volume.

Continuous flow microfluidic system

The present disclosure is directed towards a disposable microfluidic cartridge configured for use in a system for the small scale production of nanoparticles used in scientific research or therapeutic applications. The system can be used to produce a wide variety of nanoparticles, including but not limited to lipid and polymer nanoparticles, carrying a variety of payloads. The system provides for a simple workflow which in certain embodiments can be used to produce a sterile product.

Disposable microfluidic cartridge

Transfection reagent composition, lipid nanoparticles prepared from the transfection reagent composition, kits that include the transfection reagent composition, and methods for making and using lipid nanoparticles prepared from the transfection reagent composition. Lipids when dispersed in aqueous media readily form liposomes, such as unilamellar vesicles and multilamellar vesicles. Liposomes have been used successfully to encapsulate and deliver a wide range of chemicals including nucleic acids, proteins and small molecule drugs, to cells.

Lipid nanoparticles for transfection and related methods

Methods and devices for making particles at small volumes.

Device for formulating particles at small volumes

In various embodiments, the present invention provides zwitterionic lipids, encapsulants incorporating these zwitterionic lipids and such encapsulants encapsulating one or more bioactive agent. An exemplary bioactive agent is a nucleic acid. Also provided are pharmaceutical formulations of the encapsulants and methods of using such formulations to deliver a bioactive agent to a subject in treating or diagnosing disease in that subject.

Zwitterionic lipids

Growing community of inventors

Belmont, CA, United States of America

Colin Walsh