University of Delaware

Colin Thorpe

Joseph M Fox

Yixin Xie

Yinzhi Fang

Xinqiao Jia

Han Zhang

Joel Rosenthal

Jennifer Jaje

Wenzhong Wang

Will Trout

Amanda M Tallon

William Trout

Ashlyn S Cantrel

Shuang Liu

Julia E Rosenberger

A method for catalytically converting a dihydrotetrazine 1 into a tetrazine 2, wherein the dihydrotetrazine 1 comprises a first R group and a second R group, wherein the first R group is a substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, or heteroatom-containing group, and the second R group is selected from the group consisting of H and substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, and heteroatom-containing groups; wherein the method comprises (a) providing the dihydrotetrazine 1 in a reaction medium, and (b) adding an enzyme as a catalyst and an oxidant to the reaction medium, whereby the dihydrotetrazine 1 is converted to the tetrazine 2.

Methods for inducing bioorthogonal reactivity

A method for catalytically converting a dihydrotetrazine 1 into a tetrazine 2, wherein one R group on the dihydrotetrazine 1 is a substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, or heteroatom-containing group, and the other R group is selected from the group consisting of H and substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl,- or heteroatom-containing groups; 1, 2 wherein the method comprises oxidizing dihydrotetrazine 1 in a reaction medium in the presence of a catalyst and a stoichiometric oxidant. 

Methods for isolating a highly purified QSOX enzyme from milk or whey are described. The enzyme may be used to generate intermolecular and intramolecular disulfide bridges and to facilitate oxidative protein folding.

Isolation of quiescin-sulfhydryl oxidase from milk

Acyl-CoA dehydrogenase allenic inhibitors

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Newark, DE, United States of America

Colin Thorpe