Zymergen Inc.

Colin Scott Maxwell

Shawn Szyjka

Kedar Patel

Sara Da Luz Areosa Cleto

Philip Weyman

Aaron Miller

Sharon Nademanee

Elizabeth Lauren Meier

Solomon Henry Stonebloom

Stephen Blaskowski

Cameron Coates

Melissa Netwal

The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.

Iterative genome editing in microbes

The present invention relates to methods for editing the genome of a microbial host cell in one or more rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in a pooled and/or iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Compositions and kits for performing the methods are also disclosed.

Pooled genome editing in microbes

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Emeryville, CA, United States of America

Colin Scott Maxwell