British Technology Group Limited

United States of America, as Represented by the Secretary of Agriculture

Colin R Young

Philip D Minor

Jeffrey William Almond

Michael A Skinner

Larry H Stanker

Ross C Beier

Hybridoma cell lines have been generated which produce and secrete monoclonal antibodies which selectively bind to 4,4&prime;-dinitrocarbanilide (DNC), the active agent of nicarbazin. These hybridomas may be obtained by using as an immunization agent or immunogen, p-nitroaniline which has been conjugated to an immunogenic carrier. DNC in biological samples may be detected and quantified by contacting the sample with the antibodies to form a DNC/antibody immunocomplex when DNC is present, which immunocomplex may then be detected. The monoclonal antibodies also may be incorporated into kits for the detection and quantification of DNC and/or nicarbazin.

Monoclonal antibodies to 4,4&prime;-dinitrocarbanilide and a method for analyzing for the drug nicarbazin

An attenuated enterovirus or rhinovirus, suitable for use as a vaccine, has a reversed base pairing in the part, or in a part corresponding to the part, of the 5' non-coding region of the genome of poliovirus type 3 Leon strain shown below: ##STR1## A suitable attenuated poliovirus has the bases G and C at positions 469 and 534 respectively for a type 1 or type 2 poliovirus or at positions 472 and 537 respectively for a type 3 poliovirus.

Attenuated viruses

Attenuated polioviruses

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College Station, TX, United States of America

Colin R Young