Global Life Sciences Solutions USA LLC

Ge Healthcare Bio-sciences Corp.

Cytiva Sweden Ab

Other

General Electric Company

Colin R Tuohey

Richard Lee Damren

Thomas John Erdenberger

Parrish M Galliher

Jonathan A Kenney

Michael Edward Fisher

Joseph D Crowell

Ralph Stankowski

Patrick M Guertin

Kenneth P Clapp

Patrik Berndt Akerstrom

Klaus Gebauer

Mats Olsson

Steven James Forsberg

Anindya Kanti De

Anindya Sengupta

Nirmal Kumar Thanikachalam

Wei Jin

David Lawrence Brazell

Nagaraj Raghavendra Rao

John Swibes

Roberto Miguel Cabral

Sampath Telikcherla Kandala

Peter A Mitchell

Laura E Niklason

Geoffrey L Hodge

Patric Fricking

Andreas Castan

Justin T Strader

Ethan Michael Barrieau

Eric Tenander

William E Tente

Shannon Dahl

Juliana Blum

Alexander Mark Kilar

Patrick Guertin

Parish M Galliher

Ted Deloggio

Jiyoung Lee

Michelle Ann Parziale

Elizabeth Stegner

Manas Dash

Manus Dash

Timothy Becker

Michelle Parziale

Kandakumar Murugesan

Alexander Kilar

Prasad Bagiyanathan

Sairam Cherunni

Nikhil Chandramouli

Timothy J Becker

Nikhil Vagga Chandramouli

Sampath Telikherla Kandala

Peter Mitchell

The present invention relates to a bioprocess bagcomprising a bag wall defining an enclosed volume for holding biomaterials. The bag wall comprises at least one inlet portand at least one outlet port. The bioprocess bagalso comprises a tube structurecomprising a first opened-end proximate the bag wall and a second distal end, the tube extending into the enclosed volume, and the tube structurecomprising a reinforced portionproximate the first opened-end.

Reinforced component for cell cultivation bioreactor

A support housing for a flexible bioprocess bag, the housing comprising at least one side wall having a first segment and a second segment, the first segment movable in relation to the second segment between an open and a closed position, wherein at least a supporting part of the housing is translatable from an operating position to a bag loading position, wherein the supporting part comprises one or more retainers for holding the flexible bioprocess bag upon re-translation of the supporting part to the operating position and movement of the first segment to the closed position, and wherein the first segment comprises a first drive unit for connecting and driving a first mixing unit in the flexible bioprocess bag.

Housing for holding a flexible bioprocess bag and method for assembling the bag in the housing

Disclosed herein is a single use continuous recovery, flow-through harvest vessel and corresponding method for harvesting culture medium and simultaneously either leaving the microcarrier beads behind in the vessel or flowing microcarrier beads and medium back into a bioreactor.

Continuous recovery harvest bag

A flexible bioprocess bag comprising a number of flexible panels which are sealed to each other such that when the bag is filled they form at least a bottom of the bag and a side surface of the bag, wherein one of the flexible panels is called a bottom panel and when the bag is filled said bottom panel will constitute the bottom of the bag and parts of the side surface of the bag, said parts of the side surface being bent side parts of the bottom panel.

Flexible bag

A connection clamping device () arranged for securing a flexible tube () to a barbed end () of a tube connector (), whereby the barbed end () comprises a barb () and the tube connector during connection is arranged to protrude into an end of the flexible tube (), characterized in that said connection clamping device comprises two sections () which, during connection when the tube connector protrudes into an end of the flexible tube, are arranged to be connected and locked to each other by a locking mechanism () around the tube connector () and the flexible tube (), such that the flexible tube () is compressed against the tube connector () by a rib () provided on the inner circumference of the connection clamping device.

Connection clamping device

A bioprocess mixer (), which comprises: &#x2014;a support vessel () with at least one side wall () and a bottom wall (), where the walls define a support vessel inner volume (), and at least a first () and a second () magnetic impeller drive unit; and &#x2014;a flexible bag () adapted to fit inside the support vessel inner volume, where the bag has at least one bag side wall (), a bag bottom wall () and a bag top wall () defining a bag inner volume (), and at least a first () and a second () magnetic impeller rotatably attached to a bag wall in the bag inner volume.

Bioprocess mixer

An apparatus for draining a bioreactor vessel includes a tubular body portion having an interior passageway, and at least one aperture in the tubular body portion providing for fluid communication with the interior passageway, the tubular body portion being configured for positioning at a bottom of a vessel, and a suction tube having a first end configured for fluid coupling with the tubular body portion, and a second end configured for fluid coupling with a port in a sidewall of the vessel.

System, method and apparatus for draining a bioreactor vessel

Housing for holding a flexible bioprocess bag

A probe assembly for inserting a probe into a flexible or semi-rigid object is provided. The probe assembly includes an aseptic connector for coupling to the object, a probe sheath that extends longitudinally from the aseptic connector, and an actuator for deploying the probe within the object. The probe sheath has a plurality of concentric sheath elements and an inner longitudinal volume that is configured to permit longitudinal movement of the probe within the probe sheath. The actuator moves the probe longitudinally within the probe sheath by telescoping a first sheath element of the plurality within another sheath element of the plurality such that the probe is moved to a position sufficient to measure at least one parameter within the object.

Probe assembly

The invention discloses a bioreactor apparatus () for cultivation of cells comprising: a) a disposable bioreactor vessel () with one or more walls () defining an inner volume (), at least one port () in a wall, wherein the disposable bioreactor vessel is positioned in a rigid support structure (); and b) a heater (), capable of heating an amount of culture medium to a target temperature in the range of 55-95&#xb0; C., while the amount of culture medium is being confined in or conveyed to the inner volume.

Inactivation of viruses

Disclosed herein is a system and method for condensing moisture in a gas stream entering or leaving a bioreactor, the system comprising: a contact condenser container fluidically coupled to the bioreactor through an exhaust line; a condensate accumulator fluidically coupled to the contact condenser container through at least a first condensate line and a second condensate line; the condensate accumulator further fluidically coupled to the bioreactor through a condensate overflow line; a first condensate control device disposed on the first condensate line and configured to control a flow of condensate leaving the contact condenser container and entering the condensate accumulator; and a second condensate control device disposed on the second condensate line and configured to control a flow of condensate leaving the condensate accumulator to be mixed with the gas stream.

System and method for condensing moisture in a bioreactor gas stream

The invention discloses a method for virus clearance of a cell culture medium, comprising the steps of: i) providing a bulk medium portion, comprising amino acids and glucose, and a first additive portion, comprising vitamins in aqueous solution; ii) subjecting the bulk medium portion to a high temperature short time treatment (HTST); iii) passing the first additive portion through a virus retentive filter or an ultrafilter; and iv) after steps ii) and iii), mixing the bulk medium portion with the first additive portion to obtain a cell culture medium.

Method for virus clearance

A bioreactor system and packaging is provided. The bioreactor system includes a vessel for housing biomaterials for processing and a support structure. The vessel includes a flexible material defining a chamber and a mixing system positioned within the chamber. The mixing system includes an agitator for imparting motion and mixing to the contents of the vessel and includes a base affixed to the flexible material at a base section of the chamber, a shaft moveably mounted in the base and extending from the base into the chamber and at least one mixing element mounted to the shaft, the shaft configured to be driven by a motor magnetically coupled to the shaft and external to the lower portion of the chamber. The support structure is connected to the mixing system such that the shaft is moveable therein and configured to cooperate with an external structure to provide support for the shaft.

Disposable container, mixing system and packaging

Growing community of inventors

Marlborough, MA, United States of America

Colin R Tuohey