Pamgene Bv

Pamegene B.v.

Colin John Ingham

Maria Helena Hilhorst

Herman Blok

Hendrik Sibolt Van Damme

Richard Michael Anthony

Dirk Van Alewijk

The present invention relates to methods for integrated ribonucleic acid integrity assessment and analysis comprising the steps of: (a) providing a support having immobilized thereon a set of detector probes and a set of at least two control probes, wherein (i) a first of said at least two control probes is complementary to 23S or 28S rRNA or a functionally equivalent RNA, and (ii) a second of said at least two control probes is complementary to 16S or 18S10 rRNA or a functionally equivalent RNA; (b) contacting said support with analyte ribonucleic acids derived from a sample, under conditions allowing hybridisation of complementary analyte ribonucleic acids and immobilized probes to form analyte ribonucleic acid/probe complexes; wherein said hybridisation generates a detectable signal; 15 (c) detecting signals generated by said complexes; (d) determining the ratio of signals generated by the 28S/control probe and 18S rRNA/control probe or 23S rRNA/control probe and 16S rRNA/control probe or functionally equivalent rRNA/control probe complexes; (e) assessing the integrity of said sample; and 20 (f) evaluating microarray analysis results in view of said integrity assessment. The present invention further relates to use of said methods as well as microarrays, devices comprising said microarrays, kits for carrying out said methods and computer products.

Method and device for integrated nucleic acid integrity assessment and analysis

The present invention relates to methods for screening of cellular responses of cellular components comprising: (a) providing cellular components on the surface of a substrate, said substrate having immobilized thereon an array of detector molecules; (b) delivering test compounds to positions on the substrate corresponding to the arrayed detector molecules on the surface of said solid substrate; (c) incubating said test compounds with said cellular components on the surface of the solid support, under conditions allowing the induction of cellular responses; (d) assaying said cellular responses; and, identifying and characterizing the cellular responses induced by said test compounds. The present invention further relates to the uses of said methods as well as microarrays and kits for carrying out said methods.

Method for high throughput cell-based assays using versatile living microarrays

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Den Bosch, Netherlands

Colin John Ingham