New Zealand Institute for Crop & Food Research Limited

The New Zealand Institute for Plant and Food Research Limited

Colin Charles Eady

Carolyn Elizabeth Lister

Fernand Othmar Kenel

Sheree Alma Brinch

This invention relates to methods for the transformation of plants from the genus, and transformed plants produced according to the method. Specifically, this invention relates to direct transformation ofleaf tissue usingmediated transformation, and plants regenerated from the transformed leaf tissue. In various aspects, the invention relates to a method of obtaining a transformedleaf tissue and methods of obtaining a transformedplant by regenerating the transformed leaf tissue.

Transformation and regeneration of allium plants

The invention relates to a novel transformation method for plants of the genus. Plants transformed by the method are also provided. The method preferably involves an-mediated transformation, involving the transformation of immature embryos as the explant source and wherein the method is carried out without passage through the callus phase.

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The invention relates to a novel transformation method for plants of the genus, in particular to onion plants. Plants transformed by the method are also provided. The method preferably involves an-mediated transformation, more preferably involving immature embryos as the explant source and employing a binary vector.

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Christchurch, New Zealand

Colin Charles Eady