Centre National De La Recherche Scientifique

Colette Delavier

Stefano Marullo

Laurent Jean Emorine

Donny Strosberg

The invention relates to a vector capable of being replicated in cultures of unicellular organisms, this vector containing a gene coding for a eukaryotic protein having the biological activity of a membrane receptor and interacting with a regulatory protein&mdash;called the G protein&mdash;able to bind molecules of guanosine triphosphate (GTP). The invention also relates to cell organisms transformed by the above vectors. It also relates to procedures for the detection of the capacity of a molecule to behave as a ligand for a receptor and a procedure for studying the affinity of a receptor for a ligand as well as a kit for detecting the possible affinity of a ligand for a receptor.

VECTOR MODIFIED BY SEQUENCE CODING FOR A SEQUENCE OF AMINO ACIDS CONTAINED IN A MAMMALIAN PROTEIN HAVING THE BIOLOGICAL ACTIVITY OF A MEMBRANE RECEPTOR, EXPRESSION OF THIS SEQUENCE IN TRANSFORMED UNICELLULAR CULTURES BY MEANS OF THIS VECTOR, PROCEDURE FOR STUDYING LIGANDS RECOGNIZING THESE RECEPTORS

The invention relates to a vector capable of being replicated in cultures of unicellular organisms, this vector containing a gene coding for a eucaryotic protein having the biological activity of a membrane receptor and interacting with a regulatory protein--called the G protein--able to bind molecules of guanosine triphosphate (GTP). The invention also relates to cell organisms transformed by the above vectors. It also relates to procedures for the detection of the capacity of a molecule to behave as a ligand for a receptor and a procedure for studying the affinity of a receptor for a ligand as well as a kit for detecting the possible affinity of a ligand for a receptor.

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Paris, France

Colette Delavier