Oxford Nanopore Technologies Limited

Caris Science, Inc.

Caris Life Sciences Switzerland Holdings Gmbh

Pronota N.v.

Clive Gavin Brown

James White

Andrew John Heron

James Anthony Clarke

Daniel John Turner

Gavin Harper

John Milton

Rebecca Victoria Bowen

Stuart William Reid

Jason Robert Hyde

Graham Hall

James Peter Willcocks

Lakmal Nishantha Jayasinghe

Elizabeth Jayne Wallace

Koen Kas

Christopher Peter Youd

Paul Raymond Mackett

Sven Agnes Jan Eyckerman

Mark Bruce

Richard George Hambley

Jonathan Bankes Pugh

Daniel George Fordham

Pedro Miguel Ortiz Bahamon

Neil Roger Wood

Joseph Hargreaves Lloyd

Christopher James Wright

Daniel Ryan Garalde

Szabolcs Soeroes

Luke McNeill

David Jackson Stoddart

Domenico Caprotti

Gurdial Singh Sanghera

David Alves

Justin Millis

Ken Healy

Richard Alexander Gutierrez

James Edward Graham

Roger Charles Gill

Mark David Jackson

Jonathan Edward McKendry

Richard Kenneth John Wiltshire

Antonino Puglisi

Ping Xie

David Antoni Alves

Mark Hyland

Rhodri Rhys Davies

Robert Greasty

Mark John Bruce

Luke Mcneill

Andy Heron

Rebecca Bowen

Stuart Reid

Lakmal Jayasinghe

Ioana Pera

An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.

Formation of array of membranes and apparatus therefor

An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.

Biochemical analysis instrument

A sequence of polymer units in a polymer (), eg. DNA, is estimated from at least one series of measurements related to the polymer, eg. ion current as a function of translocation through a nanopore (), wherein the value of each measurement is dependent on a k-mer being a group of k polymer units (). A probabilistic model, especially a hidden Markov model (HMM), is provided, comprising, for a set of possible k-mers: transition weightings representing the chances of transitions from origin k-mers to destination k-mers; and emission weightings in respect of each k-mer that represent the chances of observing given values of measurements for that k-mer. The series of measurements is analysed using an analytical technique, eg. Viterbi decoding, that refers to the model and estimates at least one estimated sequence of polymer units in the polymer based on the likelihood predicted by the model of the series of measurements being produced by sequences of polymer units. In a further embodiment, different voltages are applied across the nanopore during translocation in order to improve the resolution of polymer units.

Method and system of estimating a sequence of polymer units

The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.

Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores

The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.

Coupling method

The present invention is a method for measuring the amount of at least one molecule in a biological sample, the method comprising a) combining the sample, or a derivative thereof, with one or more aptamers and allowing one or more molecules in the sample to bind to the aptamer(s); b) separating bound from unbound molecules; and c) quantifying the molecule(s) bound to the or each aptamer, wherein quantification of the bound molecule(s) is carried out by sequencing at least part of the or each aptamer. Uses of and products derived from the method are also contemplated.

Use of aptamers in proteomics

Method of operating a measurement system to analyze a polymer

The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.

Method for nucleic acid detection by guiding through a nanopore

The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein. The invention also relates to improved transmembrane pores and polynucleotide binding proteins.

Method of improving the movement of a target polynucleotide with respect to a transmembrane pore

The invention relates to new methods of attaching one or more polynucleotide binding proteins to a target polynucleotide. The invention also related to new methods of characterising target polynucleotides.

Method for attaching one or more polynucleotide binding proteins to a target polynucleotide

The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.

Aptamer method

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Cambridge, United Kingdom

Clive Gavin Brown