Children's Medical Center Corporation

Harvard College

The State University of New York

Clinton H Hansen

Wesley Philip Wong

Darren Yang

Andrew Ward

Mounir Ahmad Koussa

Kenneth Anders Halvorsen

Eric M Mulhall

Hongyu Zhao

Johanna Blass

Arun Richard Chandrasekaran

Provided herein are products and methods for detecting analytes using polymers that bind to such analytes and thereby undergo a conformational change or contribute to a newly formed complex.

Method for detection of analytes via polymer complexes

This disclosure provides methods for generating functionalized nanoswitches, as well as the functionalized nanoswitches themselves, and methods of use thereof.

Nucleic acid nanoswitch construction methods

Provided herein, inter alia, are improved methods for detecting analytes, including proteins and nucleic acids. In some instances, the analytes are detected in complex matrices such as serum.

Rapid and sensitive detection and quantification of analytes in complex samples using polymer-based methods

Provided herein are nucleic acid-based nanoswitches that can detect specific nucleic acids and other analytes types by for example a simple gel electrophoresis readout. Binding of the target to the nanoswitches induces a conformation change from a linear, open conformation to a looped, closed conformation. These nanoswitches may be used in diagnostic assays such as nucleic acid-based diagnostic assays, to detect, measure and/or purify a variety of targets including low abundance targets.

Compositions and methods for analyte detection using nanoswitches

Provided herein are methods and products for detecting analytes in a sample. The analytes may be rare analytes such as biomarkers in a biological sample. These methods make use of nucleic acid nanoswitches that adopt a particular conformation and have a particular length in the presence of an analyte.

Force-controlled nanoswitch assays for single-molecule detection in complex biological fluids

The invention provides compositions comprising nucleic acid complexes for use in screening compounds based on their ability to modulate binding interactions, wherein the compounds are barcoded.

Nucleic acid complexes for screening barcoded compounds

Systems and apparatus for performing analysis of human biological samples are described. Collected biological samples are combined with a substance including nucleic acid complexes and the combined substance is processed using gel electrophoresis. An indication of a detected compound in the biological sample and/or a particular medical condition associated with the detected compound is output from the apparatus.

Growing community of inventors

Cambridge, MA, United States of America

Clinton H Hansen