Inscripta, Inc.

Clint Davis

Eileen Spindler

Charles D Johnson

Tian Tian

Phillip Belgrader

Isaac David Wagner

Julia Swavola

The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.

Detection of nuclease edited sequences in automated modules and instruments

The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of 'edit or die.' Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome.

Methods for increasing observed editing in bacteria

The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells.

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Boulder, CO, United States of America

Clint Davis