Pioneer Hi-bred International, Inc.

Clifford Paul Hunter

Roger Monk

Maria Laura Mayor

Yue Yun

Cleve Douglas Franks

Xinli Emily Wu

Justin Schares

Randal Arnold

Matthew Paul Cope

Justin M Gifford

Roberto Barreiro

Rex Delong

Tanveer Hussain

John Robert Jaster

Justin Andrew Schares

Two sorghum maternal haploid inducer lines SMHI01 and SMHI02 are provided including seed, plants and plant parts thereof. Methods for producing sorghum haploid embryos using SMHI01 and SMHI02 are also provided. The sorghum haploid embryos produced as a result of the use of either maternal haploid inducer line SMHI01 or SMHI02 may be doubled to produce doubled haploid embryos, seeds, and plants as part of a sorghum breeding program.

Sorghum maternal haploid inducing line SMHI02

Twomaternal haploid inducer lines SMHI01 and SMHI02 are provided including seed, plants and plant parts thereof. Methods for producinghaploid embryos using SMHI01 and SMHI02 are also provided. Thehaploid embryos produced as a result of the use of either maternal haploid inducer line SMHI01 or SMHI02 may be doubled to produce doubled haploid embryos, seeds, and plants as part of abreeding program.

Sorghum maternal haploid inducing line SMHI01

Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling,-mediated transformation, or herbicide selection as part of an embryo rescue process.

Methods and devices for creating doubled haploid embryos using oil matrices

Methods for creating doubled haploid maize embryos using oil matrices

Growing community of inventors

Mililani, HI, United States of America

Clifford Paul Hunter