Illumina Corporation

Other

Illumina Cambridge Limited

Illumina Singapore Pte. Ltd.

Clifford Lee Wang

Tarun Kumar Khurana

Andrew Antony Brown

Wayne N George

Yir-Shyuan Wu

Jeffrey S Fisher

Fiona Kaper

Mathieu Lessard-Viger

Tong Liu

Yin Nah Teo

Hongxia Xu

Xiangyuan Yang

Burak Okumus

Victor J Quijano

Hassan Bohra

Eric Brustad

Pietro Gatti Lafranconi

Dan Cao

Shi Min Xiao

Maria Rogert Bacigalupo

In an example, a target material is immobilized on two opposed sequencing surfaces of a flow cell using first and second fluids. The first fluid has a density less than a target material density and the second fluid has a density greater than the target material density; or the second fluid has a density less than the target material density and the first fluid has a density greater than the target material density. The first fluid (including the target material) is introduced into the flow cell, whereby at least some of the target material becomes immobilized by capture sites on one of the sequencing surfaces. The first fluid and non-immobilized target material are removed. The second fluid (including target material) is introduced into the flow cell, whereby at least some of the target material becomes immobilized by capture sites on another of the sequencing surfaces.

Immobilization in flow cells

An example of a biotin-streptavidin cleavage composition includes a formamide reagent and a salt buffer. The formamide reagent is present in the biotin-streptavidin cleavage composition in an amount ranging from about 10% to about 50%, based on a total volume of the biotin-streptavidin cleavage composition. The salt buffer makes up the balance of the biotin-streptavidin cleavage composition. In some examples, the biotin-streptavidin cleavage composition is used to cleave library fragments from a solid support. In other examples, other mechanisms are used to cleave library fragments from a solid support.

Biotin-streptavidin cleavage composition and library fragment cleavage

Some embodiments of the methods and compositions provided herein relate to the selective cleavage of a target nucleic acid. Some such embodiments include the selective cleavage of a target nucleic acid that is associated with a DNA-binding protein or comprises a methylated CpG island, with a recombinant nuclease. In some embodiments, the DNA-binding protein comprises a chromatin protein. Some embodiments also include the enrichment of non-target nucleic acids in a sample by selective cleavage of target nucleic acids in the sample, and removal of the cleaved target nucleic acids from the sample.

Methods and compositions for selective cleavage of nucleic acids with recombinant nucleases

Embodiments of the methods and compositions provided herein relate to the selective cleavage of target nucleic acids. Some embodiments include recombinase-mediated selective cleavage of target nucleic acids with single-stranded nucleic acid probes and a recombinase. Some embodiments also include the enrichment of non-target nucleic acids in a sample by selective cleavage of target nucleic acids in the sample, and removal of the cleaved target nucleic acids from the sample.

Methods and compositions for recombinase-mediated selective cleavage of nucleic acids

An exercising device including a base plate and elastic straps extending from the base plate. The base plate is formed with lateral extensions on which a user can stand while stretching the elastic straps. At least one channel is provided in the base plate with at least one slot extending from the channel through the base plate. One end of each strap is connected to a block-like lug which securely fits into the channel, with the strap extending through the slot. In this way, the straps are securely held in position by the base plate. In addition to an upwardly extending vertical pair of straps, there also may be provided a horizontal pair of straps laterally extending from the base plate. One or more straps may be connected to one block-like lug, where the straps may extend in the same direction and/or at a right angle to each other.

Elastic pull type exercising device

Growing community of inventors

Redwood City, CA, United States of America

Clifford Lee Wang