University of Washington

Other

Placer Dome, Inc.

Clement E Furlong

Sinclair S Yee

Peter Kowalczyk

Peter C Kauffman

Timothy M Chinowsky

Michael S Grow

Scott D Soelberg

Scott Jorgenson-Soelberg

James B Clendenning

Jared Tritz

Noel W Kirshenbaum

Victor Chevillon

Alexei N Naimushin

Richard W Woodbury

This specification discloses various improvements in the field of SPR sensing systems. One improvement relates to a portable SPR sensing system, e.g., a system contained within a suitcase that can be hand-carried to a monitoring site. Another improvement relates to a portable, cartridge-based SPR sensing system. In this system, selected portions of the system's electrical and fluidics systems are allocated between a base unit and a removable/disposable cartridge. Other improvements relate to methods or protocols for operating an SPR sensing system. Such methods provide for the elimination of false positives and increased sensitivity, e.g., by using secondary antibodies with specificity for different target epitopes and by sensor element redundancy. In addition, protocols are provided for the detection of small molecules. Such protocols may employ a competition type assay where the presence of the analyte inhibits the binding of antibodies to surface immobilized analyte, or a displacement assay, where antibodies bound to the analyte on the sensor surface are displaced by free analyte.

Portable and cartridge-based surface plasmon resonance sensing systems

Methods for detecting gold and quantitating gold in ore samples utilizing a gold-specific protein are provided, including methods for multiple sample handling. Also provided are methods for extracting gold from mineral suspensions utilizing a magnetic mineral binding reagent and gold-specific protein, or hydrophobic reagent and gold-specific protein in conjunction with a flotation reagent.

Methods for detecting and extracting gold

A method of binding a recognition element to a gold surface (,) comprising the steps of attaching a GBP-AP protein (,) to a gold surface (,), digesting away the AP domain of the protein (,) with a proteolytic enzyme from the bound GBP domain (,) attached to the gold surface (,), and attaching a recognition element, such as Protein A (,) or an antibody,, antigen, nucleic acid or other binding partner to the GBP domain (,) which remains on the gold surface (,), is disclosed.

Versatile surface plasmon resonance biosensors

Growing community of inventors

Bainbridge Island, WA, United States of America

Clement E Furlong