Bio-rad Laboratories, Inc.

Clayton T McKee

William Strong

Christopher Belisle

Cory Panattoni

Lee Olech

Michael Griffin

Steve Swihart

Thomas R Berkelman

Emmanuel Quiroz

Mingde Zhu

Paul Liu

Lateral flow devices, methods and kits for performing lateral flow assays are provided.

Lateral flow assay device

Lateral flow device

Systems and methods for producing blot images. A blot, for example a western blot, is imaged using an imaging system having a field of view and a magnification. Features of interest in the blot correspond to features in the digital image, and the sizes of the features in the digital image depend on the magnification of the imaging system. A structuring element is selected based on the sizes and shapes of the features in the digital image, and the image is morphologically eroded and dilated varying numbers of times. The eroded and dilated image is subtracted from the original blot image to remove background signal from the blot image, producing an output image. The number of erosions needed to completely erode the features of interest is determined automatically, for example by investigating the behavior of the kurtosis of the output image as a function of the number of erosions performed.

Reduction of background signal in blot images

Gel electrophoresis receptacles and methods of using such receptacles are provided. In one embodiment, a receptacle comprises a cavity for receiving an electrophoresis gel, wherein at least a portion of the receptacle is formed from cyclic olefin copolymer or cyclic olefin polymer.

Electrophoresis receptacles and methods

Methods and compositions for imaging and scanning Western or immunoblots are disclosed herewith.

Methods and compositions for fluorescence detection

Lateral flow blotting assay

Methods, compositions, and kits for performing analyte detection in a lateral flow assay.

Compositions, methods, articles of manufacture, and kits are provided for storage and delivery of proteins.

Enhanced functionality and delivery of a protein from a porous substrate

Kits, systems, and methods are provided for transferring biological macromolecules from an electrophoresis slab gel to a blotting membrane using conductive polymer electrodes.

Dry protein transfer

Devices, systems, methods, and kits are provided for performing separation, immobilization, blotting, and/or detection of analytes from biological samples. In some embodiments, the devices are constructed from two solid substrates with surfaces in contact. The devices include a plurality of channels formed from indentations in these surfaces. The indentations can be aligned with each other across the interface between the substrates, and realigned by shifting or sliding one substrate relative to the other. In some embodiments, the devices are constructed from three layers of a solid substrate. A separation channel in the middle layer of the device is first used for analyte separation. The middle layer can then be slid relative the top and/or bottom layer, thereby aligning the separation channel with a blotting membrane. Analytes can then be transferred to the membrane using electrodes in the top and bottom layers.

Growing community of inventors

Davis, CA, United States of America

Clayton T McKee