Novo Nordisk A/s

Claus Yding Andersen

Anne Grete Byskov

Ivan Verner Diers

Erling Guddal

Henning Tho Slashedgersen

Lars Nordholm

Ole Wassmann

The present invention relates to a method for in vitro maturation of oocytes comprising the steps of: The invention also relates to an oocyte culture medium comprising a nuclear maturation inhibiting substance and comprising one or more gonadotropins and/or one or more growth factors. The nuclear maturation inhibiting substance may be a MAS antagonist or an FF-MAS synthesis inhibitor, preferably a cytochrome P450 lanosterol 14&#x3b1;-demethylase (P45014DM) inhibitor e.g. ketoconazole or 22-hydroxycholesterol. The one or more gonadotropins and/or one or more growth factors are preferably a combination of EGF and FSH and/or LH. The invention also relates to the use of a nuclear maturation inhibiting substance and one or more gonadotropins and/or one or more growth factors as described above for the preparation of a cell culture medium for in vitro maturation of oocytes.

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The present invention relates to certain sterol derivatives for use as medicaments.

Regulation of meiosis using sterols

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Copenhagen, Denmark

Claus Yding Andersen