Maxygen, Inc.

Codexis Mayflower Holdings, LLC

Codexis, Inc.

Claus M Krebber

Jeremy S Minshull

Phillip A Patten

Jon Edward Ness

Linda A Castle

Matthew B Tobin

Willem P C Stemmer

Venkiteswatan Mani Subramanian

Stephen DelCardayre

Gjalt W Huisman

Steven Henry Bass

Joseph A Affholter

Ying-Xin Zhang

Ling Yuan

Anthony Cox

Steve Bass

William P C Stemmer

Venkiteswaran Subramanian

Stephen delCardayre

Jovana Nazor

Fernando Valle

Jonathan Vroom

Louis Clark

Yong Hong Chen

Sun Ai Raillard

Kristian Karlshoej

Kenneth W Mitchell

Kaman Chan

Yoram Barak

Tony Cox

The invention generally relates to the production of a fatty alcohol composition from recombinant microbial cells. The fatty alcohols are produced by expressing a gene encoding a heterologous fatty alcohol forming acyl-CoA reductase ('FAR'); a gene encoding a heterologous thioesterase ('TE') gene and a gene encoding an acyl-CoA synthetase (&#x201c;ACS&#x201d;).

Production of fatty alcohols from engineered microorganisms

The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

Evolution of whole cells and organisms by recursive sequence recombination

Apparatus and methods for high throughput mass spectrometry are provided. The methods involve sample preparation in an off-line parallel purification system. Such methods include but are not limited to the use of an appropriate buffer when generating samples or the use of a solid support for tagged components. The samples prepared in this way do not then need to be column separated. The apparatus provided includes a cell growth plate for growing cells and generating products and/or reactants, an off-line parallel purification system, a mass spectrometer, and an automatic sampler that transports samples and injects them into the mass spectrometer of the apparatus. The methods and apparatus described are used, for example, in screening enzyme reaction pathways.

High throughput mass spectrometry

The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

The invention provides methods for the production of polynucleotides with a desired property (e.g., conferring a desired phenotype and/or encoding polypeptide with a desired property) which is selectable or can be screened for. The method includes making insertions and/or deletions at random sites in DNA segments in a population. In some embodiments the random insertions and deletions are made recursively.

Method for producing polynucleotides with desired properties

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Mountain View, CA, United States of America

Claus M Krebber