Bio-rad Laboratories, Inc.

Hercules Corporation

Roche Molecular Systems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Do, Duc

Litterst, Claudia

Maar, Dianna

Claudia Litterst

Dianna Maar

Svilen Tzonev

Duc Do

John Frederick Regan

Yann Jouvenot

Geoff McDermott

Jeremy Agresti

Austin So

Wei Yang

Luis A Ugozzoli

Ha Bich Tran

Svilen S Tzonev

Sean Chien

Xiaoying Chen

Austin P So

Ha Tran

Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.

Digital assays with a generic reporter

Provided herein are methods and compositions for performing multiplex RT-PCR to amplify short nucleic acids.

Multiplex detection of short nucleic acids

Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.

Multiplexed digital assay with specific and generic reporters

Methods compositions and kits are provided for performing a chromatin or chromosome conformation capture assay in partitions.

Chromosome conformation capture in partitions

Digital assay system, includes methods, apparatus, and compositions, for distinguishing and measuring different types of templates according to the different lengths of corresponding amplicons, which may be amplified by the same pair of primers. The system may include a length-sensitive reporter generating luminescence that varies according to amplicon length. The system may, for example, be utilized to identify, distinguish, and/or quantify wild-type and mutant/variant templates, processed and unprocessed template, a target template and a primer dimer, or the like.

Digital assays with a reporter for amplicon length

Digital assay system, including methods and apparatus, for calculating a level of one or more targets. In an exemplary method, data for amplification of a plurality of targets including a first target may be collected from partitions. A level of the first target may be calculated from only a subset of the data that excludes partitions according to target content.

Multiplexed digital assay with data exclusion for calculation of target levels

A method of characterizing telomere length. A template may be synthesized in a bulk phase reaction mixture containing genomic DNA. Partitions may be formed after synthesizing the template in the bulk phase reaction mixture, with only a subset of the partitions containing at least one copy of the template. At least a region of the template may be amplified in partitions. Amplification data may be collected from partitions. A measure of telomere length may be determined for the genomic DNA based on the amplification data. In some embodiments, the bulk phase reaction mixture may be thermally cycled for at least two cycles, and heated to a denaturation temperature and cooled to an annealing temperature in each cycle.

Digital assay for telomere length

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Walnut Creek, CA, United States of America

Claudia Litterst