Hoffmann-La Roche Inc.

Roche Diagnostics Gmbh

F. Hoffmann-La Roche Ag

Claudia Giessel

Roberto Falkenstein

Rainer Mueller

Adelbert Grossmann

Johann-Peter Thalhofer

Andreas Schaubmar

Friederike Hesse

Frank Geipel

Stephan Glaser

Hellmut Eckstein

Stephanie Ronning

Bernhard Rexer

Birgit Weydanz

Michael Ratka

Nicole Fuehrler

Marc Pompiati

Sybille Gabel

Brigitte Kraemer

Sybille Greithanner

Claus Schneider

Elvira Wolf

Thomas Kirschbaum

The current invention reports a method for purifying an immunoglobulin, wherein the method comprises applying an aqueous, buffered solution comprising an immunoglobulin in monomeric and in aggregated form to a cation exchange material under conditions whereby the immunoglobulin in monomeric form does not bind to the cation exchange material, and recovering the immunoglobulin in monomeric form from the solution after the contact with the cation exchange material.

Immunoglobulin purification

The current invention reports a method for the purification of a not-glycosylated, heterologous polypeptide, which has been recombinantly produced in a prokaryotic cell, wherein the method comprises three chromatography steps of which the first chromatography step selected from i) hydrophobic charge induction chromatography, or ii) hydrophobic interaction chromatography, or iii) affinity chromatography, or iv) ion exchange chromatography, the second chromatography step is selected from i) anion exchange chromatography, or ii) cation exchange chromatography, or iii) hydroxylapatite chromatography, or iv) hydrophobic interaction chromatography, and the a third chromatography step is selected from i) hydrophobic charge induction chromatography, or ii) anion exchange chromatography, or iii) cation exchange chromatography, or iv) hydrophobic interaction chromatography, whereby the first chromatography step is an affinity chromatography in case of polypeptides capable of interacting with metal ligands, the second chromatography step is not a hydroxylapatite chromatography step in case of polypeptides with an isoelectric point below 6.0, and the third chromatography step can be performed in flow-through mode with polypeptides having a low or high isoelectric point.

Purification of non-glycosylated polypeptides

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Bad Toelz, Germany

Claudia Giessel