Institut Für Nanophotonik Göttingen E.v.

Laser-laboratorium Goettingen E.v

Claudia Geisler

Alexander Egner

Jennifer-Rose Krueger

Francesco Rocca

Jennifer-Rose KRÜGER

For correcting aberration-induced imaging errors of an optical system which includes an objective () and an adaptive optic (), light () and a sample () are selected such that the light (), in acting upon the sample (), reduces a measurement signal () from the sample (), wherein a relative variation of the measurement signal () depends on the intensity of the light (). The measurement signal () from a focal area of the optical system in the sample () is registered over a first and a later second period of time () to determine a first measurement value and a second measurement value. Over a third period of time () which overlaps with the first and/or the second period of time, the light () is focused into the focal area by means of the optical system. A measure value for the relative variation of the measurement signal () is determined from the first and the second measurement values and used in controlling the adaptive optic () as a metric to be optimized.

Methods of correcting aberration-induced imaging errors

For multi-dimensional high-resolution imaging a structure marked with fluorescence markers, fluorescence enabling light is focused to illuminate a measurement area in a sample. A partial area of the measurement area is subjected to fluorescence inhibiting light. The partial area omits a center of the measurement area in that an intensity distribution of the fluorescence inhibiting light comprises a line-shaped intensity minimum. A minimal extension of the intensity minimum in a direction through the center area is by a factor k&#x2265;2 smaller than a diameter of the measurement area in said direction. Without spatial resolution, fluorescence light emitted out of the measurement area is measured for a plurality of consecutive angle positions of the intensity minimum about the center, while the measurement area, for each angle position, is subjected to the fluorescence enabling light. A value of the measured fluorescence light is assigned to the position of the center.

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Bad Gandersheim, Germany

Claudia Geisler