Institut National De La Sante Et De La Recherche Medicale

Institut Pasteur

Other

UniversitÉ Libre De Bruxelles

Rijksuniversiteit Groningen

Claude Parsot

Philippe J Sansonetti

Brigitte Demers

Armelle Phalipon

Laurence Arbibe

Kim Dong Wook

Adbelmounaaïm Allaoui

Anne Botteaux

Musa Sani

Egbert J Boekema

The ospF gene ofencodes a phosphatase, which is a member of a new class of phosphatases. The OspF phosphatase inhibits the activity of several proteins either by direct protein modification or transcription downregulation. These proteins include MAP kinase, IL-8, CCL20, IL-12, AP1, CREB, RPA p32, and BCL2 related proteins. Methods for treating diseases using OspF phosphatase, methods for identifying agents that modulate OspF phosphatase's activity, methods for identifying agents that mimic OspF phosphatase's activity, and immunogenic compositions comprising OspF phosphatase are provided. A strain ofcontaining an inactivated ospF gene is also provided.

Targeting the histone code as a bacterial strategy for selectively modulating gene expression

This invention relates to mutant strains of gram-negative bacteria that constitutively secrete proteins via the type III secretion machinery and to methods of identifying molecules that are able to activate or inhibit secretion in wild-type strains of gram-negative bacteria by exposing gram-negative bacterial cells to a sample molecule, wherein the bacterial cells contain a reporter gene transcriptionally fused to a promoter of a gene activated or regulated by the type III secretion machinery and detecting the presence or activity of the product of the reporter gene.

Method for screening for inhibitors and activators of type III secretion machinery in gram-negative bacteria

This invention relates to mutant strains of gram-negative bacteria that constitutively secrete proteins via the type III secretion machinery. It also relates to methods of identifying molecules that are able to activate or inhibit secretion in wild-type strains of gram-negative bacteria by exposing gram-negative bacterial cells to a sample molecule, wherein said bacterial cells contain a reporter gene transcriptionally fused to a promoter of a gene activated or regulated by the type III secretion machinery, and detecting the presence or activity of the product of the reporter gene.

Method for screening for inhibitors and activators of type III secretion

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Paris, France

Claude Parsot