Rhone-poulenc Agrochimie

Bayer Cropscience S.a.

Aventis Cropscience Gmbh

Claude Gigot

Georges L Freyssinet

Nicole Chaubet

Bernard Leroux

Michel Lebrun

Richard Derose

Alain Sailland

Richard De Rose

Nicole Chaubet, Legal Reprsentative

Rossitza Atanassova

An isolated DNA sequence capable of serving as regulatory element in a chimeric gene which can be used for the transformation of plants is disclosed. A chimeric gene containing the DNA sequence as a regulatory element for the transformation of plants is also disclosed. The gene comprises at least, in the direction of transcription, a promoter sequence, a transgene and a regulatory element, characterized in that the regulatory element comprises an intron from the noncoding 5' region of a plant histone gene, allowing the expression of the protein in the zones undergoing rapid growth. The production of transgenic plants is also disclosed.

Chimeric gene comprising intron from histone H3.3 gene

1) Chimeric gone for conferring to plants an increased tolerance to a herbicide. 2) It comprises, in the direction of transcription, a promoter region, a transit peptide region, a sequence encoding glyphosate tolerance and a polyadenylation signal region, wherein the promoter region consists of at least one promoter of a plant histone gene enabling the expression of the herbicide tolerance protein in the regions of glyphosate accumulation. 3) Production of glyphosate-tolerant plants.

Chimeric gene for the transformation of plants

An isolated DNA sequence capable of serving as regulatory element in a chimeric gene which can be used for the transformation of plants is disclosed. A chimeric gene for the transformation of plants is also disclosed. The gene comprises at least, in the direction of transcription, a promoter sequence, a transgene and a regulatory element, characterized in that the regulatory element consists of at least one intron 1 in the noncoding 5&prime; region of a plant histone gene allowing the expression of the protein in the zones and undergoing rapid growth. The production of transgenic plants is also disclosed.

Isolated DNA sequence capable of serving as regulatory element in a chimeric gene which can be used for the transformation of plants

Isolated DNA sequence which can serve as terminator region in a chimeric gene capable of being used for the transformation of plants

The present invention relates to a chimeric gene for conferring to plants an increased tolerance to a herbicide. This chimeric gene comprises, in the direction of transcription, a promoter region, a transit peptide region, a sequence encoding glyphosate tolerance and a polyadenylation signal region, wherein the promoter region consists of at least one promoter of a plant histone gene enabling the expression of the herbicide tolerance protein in the regions of glyphosate accumulation. The present invention further provides vectors containing the present chimeric genes, of glyphosate-tolerant plants.

The present invention relates to a chimeric gene for conferring to plants an increased tolerance to a herbicide. This chimeric gene comprises, in the direction of transcription, a promoter region, a transit peptide region, a sequence encoding glyphosate tolerance and a polyadenylation signal region, wherein the promoter region consists of at least one promoter of a plant histone gene enabling the expression of the herbicide tolerance protein in the regions of glyphosate accumulation. The present invention further provides vectors containing the present chimeric genes, as well as plant cells and plants transformed with such vectors which permit production of glyphosate-tolerant plants.

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Strasbourg, France

Claude Gigot