Accuri Cytometers, Inc.

Clare E Rogers

Collin Anderson Rich

Jack T Ball

A method for detecting fluorochromes in a flow cytometer, including: receiving a sample including particles each tagged with at least one of a first fluorochrome and a second fluorochrome, in which the first and second fluorochromes having distinct spillover coefficients; detecting the particles, including detecting the first and second fluorochromes with a first detector and a second detector; forming a data set for detected particles based on the detection of the first and second fluorochromes; characterizing a detected spillover coefficient for each detected fluorochrome from the data set; and sorting the detected particles into predicted fluorochrome populations based on the detected spillover coefficients. A system for detecting fluorochromes in a flow cytometer, including a flow cell, a fixed gain detection system, and a processor that generates a detected spillover coefficient for each detected particle and sorts the detected particle into predicted fluorochrome populations based on the detected spillover coefficient.

Method and system for detecting fluorochromes in a flow cytometer

A system for a flow cytometer that collects data for a sample prepared with a plurality of fluorochromes that includes a fixed gain detection system that collects data for a plurality of fluorescence channels, fluorochrome compensation factors for a plurality of fluorochromes types, and a computer system that has an interface that gathers fluorochrome information of the sample and an analysis program that compensates for spectral spillover in the collected data. The fixed gain detection system preferably has a wide dynamic range. A fluorochrome compensation factor preferably remains constant for a fixed gain detection system. The analysis program preferably uses the fluorochrome compensation factors to compensate for spectral spillover.

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Plymouth, MI, United States of America

Clare E Rogers