Michigan State University

Claire Vieille

J Gregory Zeikus

Pil Sung Kim

Maris Laivenieks

Joseph G Zeikus

Karla I Ziegelmann-Fjeld

Dinlaka Sriprapundh

Seung Hoon Song

Nucleic acids encodingmannitol dehydrogenase and themannitol dehydrogenase polypeptide are disclosed. Further provided are an electrochemical bioreactor system and a bioreactor electrode that can be used to convert glucose or fructose to mannitol.

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The present invention relates to compositions and methods utilizing thermostable and novel alcohol dehydrogenase enzymes for biosynthesizing chiral specific molecules for use as precursor molecules in synthesizing pharmaceutical compounds. Particularly, in preferred embodiments, the invention relates to directed engineering of an enzymatic catalytic site of an alcohol dehydrogenase enzyme gene for enhancing enantioselectivity for (S)-enantiomer substrate catalytic activity for providing aryl (S)-enantiomer products in stereomeric excess.

Molecular design of thermostable alcohol dehydrogenase for synthesis for chiral aromatic alcohols

The present invention provides novel isolated polynucleotides encoding a novel xylose isomerases capable of catalyzing the conversion of glucose to fructose and nucleic acids encoding for such isomerase. The present invention also provides a method of producing the xylose isomerase enzymes employing DNA encoding for the enzymes, plasmids containing the DNA, and bacteria into which the plasmids have been inserted and which produce the enzymes.

Anplasmid vector which provides a means to overexpress proteins in. The plasmid can be transformed efficiently by electroporation, and replicates in a stable manner in. The plasmid comprises at least one marker gene, operably linked to a first promoter functional in, an origin of replication functional in, a second promoter isolated from, and a cloning site downstream from the second promoter. Plasmids pLGZ901, pLGZ920, pLGZ921, and pLGZ922 are disclosed. The pckA gene polypeptide sequence and nucleic acid sequence of, including the promoter and ribosome binding site, is disclosed. Furthermore, a method for producing a recombinantis described, including a method of transformation. Additionally, a recombinantis disclosed and a method for producing succinate utilizing this recombinantis described.

Anplasmid vector which provides a means to overexpress proteins inThe plasmid can be transformed efficiently by electroporation, and replicates in a stable manner inThe plasmid comprises at least one marker gene, operably linked to a first promoter functional inan origin of replication functional ina second promoter isolated fromand a cloning site downstream from the second promoter. Plasmids pLGZ901, pLGZ920, pLGZ921, and pLGZ922 are disclosed. The pckA gene polypeptide sequence and nucleic acid sequence ofincluding the promoter and ribosome binding site, is disclosed. Furthermore, a method for producing a recombinantis described, including a method of transformation. Additionally, a recombinantis disclosed and a method for producing succinate utilizing this recombinantis described.

The present invention describes the cloning and expression of a hyperthermostable xylose isomerase gene in an industrial host and the heat facilitated purification of the enzyme. The hyperthermostable enzyme has unique features sought by the fructose syrup industry.

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Lansing, MI, United States of America

Claire Vieille