L'oreal

Claire Marionnet

Bruno Bernard

Françoise Bernerd

Peggy Sextius

Francoise Bernerd

The present invention concerns a method for evaluating the epidermal homeostasis of the skin of a subject, comprising differential analysis of the expression of a gene, preferably of two genes, selected from the group of genes consisting of KRT6B, KRT16, ESRRA, CBX3, MAP3K5, TRIO, PIK3CD, TCRB, PRRG2, ICP22BP, GPX3, GBA, BPGM, NID1, SMT3H2, LGALS2, DOC1, PRKCG, S100A8, S100A9, S100A2, S100A7, K15, SPRR1B and GSN in response to a physical or chemical challenge of the stratum corneum. It also concerns various methods for diagnosing the state of the epidermis of an individual and methods for detecting and screening products or procedures which can improve epidermal homeostasis. The invention also pertains to kits and arrays which can be used in these various methods and processes.

Molecular signature representative of dysfunctions in epidermal homeostasis

The present invention concerns a method for determining the state of epidermal differentiation of a human keratinocyte in a sample of human epithelium, comprising determining the level of expression of at least one miRNA selected from hsa-miR-455-3p, hsa-miR-141, hsa-miR-148a, hsa-miR-182, hsa-miR-224, hsa-miR-26a, hsa-miR-26b, hsa-miR-361-5p, hsa-miR-425, hsa-miR-92b, hsa-let-7i, hsa-miR-22, hsa-miR-221, hsa-miR-222, hsa-miR-29a, hsa-miR-29b, hsa-miR-663, hsa-miR-30a and hsa-miR-30c within said keratinocyte and comparing the level of expression of the selected miRNA or miRNAs with the mean level of expression of the selected miRNA or miRNAs in non-differentiated keratinocytes, or in differentiated epidermal keratinocytes, preferably originating from the same strain or from the same population. The present invention also concerns various uses of the method for determining the state of epidermal differentiation as well as pharmaceutical or cosmetic compositions.

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Ville d'Avray, France

Claire Marionnet