Merck Sharp + Dohme Corp.

Istituto Di Richerche Di Biologia Molecolare P. Angeletti S.p.a.

Cinzia Traboni

Steven W Ludmerer

Osvaldo A Flores

Donald J Graham

Robert L LaFemina

Maura Pizzuti

The present invention features methods for enhancing the ability of a genotype 2b NS5B sequence to function in a replicon, for producing replicons containing a functional genotype 2b NS5B, and for using replicons to measure the ability of a compound to affect HCV replication that is sustained with the genotype 2b polymerase. Also featured is a genotype 1b NS4B adaptive mutation. The ability to produce replicons containing a functional genotype 2b NS5B is illustrated by the production of chimeric replicons based on HCV genotype 1b where substantially all the NS5B sequence is replaced with a genotype 2b NS5B.

Growing community of inventors

Rome, Italy

Cinzia Traboni