Monsanto Technology LLC

G.d. Searle & Company

Inguran, LLC

Pharmacia Corporation

Pharmacia Corporation (of Pfizer, Inc.)

Cindy L Ludwig

David Louis Brown

John Jeffrey Talley

Matthew James Graneto

Kevin Lee Deppermann

Balekudru Devadas

Srinivasan Raj Nagarajan

Brian J Martinell

Suzanne S Metz

Stephen R Bertenshaw

Jeffery S Carter

Edward James Williams

Jerald D Heise

Angela Ranae Koestel

Mark G Obukowicz

Cathleen Elizabeth Hanau

Beth Jo Calabotta

Erik D Dersch

Donald J Rogier

Susan J Hartmann

Donald E Korte

Jeffrey Alan Graham

Gary Durack

Jeffrey A Graham

E Ann Hallinan

Lijuan Jane Wang

Francis J Koszyk

Donald J Rogier, Jr

Bruce C Hamper

Li Huang Xing

Jeremy T Hatcher

Jeffrey D Wallace

Niraj V Nayak

Gary P Vandre

Lon A Westfall

Kathleen S Crowley

Muhammad Anzar

John M Molyneaux

Bradley Didion

Steven W Kramer

Yi Yu

Karl W Aston

Renee Huff

Jeanette A Glaenzer

Subo Laio

Angela Deprow

Teresa Fletcher

James Kiefer

David Limburg

John McCall

Susan J Hartman

Charles N Graves

Jeffry Carter

John Springer

David R Helbing

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

Preparation and use of plant embryo explants for transformation

Apparatus for the preparation and use of plant embryo explants for transformation

A method of cryopreserving sperm cells which may include cooling the quantity of sperm cells to a holding temperature, maintaining the sperm cells substantially at the holding temperature for a period, and supercooling the sperm cells by cooling the sperm cells at a first cooling rate to a temperature that approaches a critical temperature zone at which ice crystal formation and changes in osmotic pressure damage sperm cells and then cooling the sperm at a second cooling rate faster than said first cooling rate through said critical temperature zone.

Methods for processing sperm cells

A method of processing animal sperm cells that includes: collecting sperm cells from a male animal; sorting the sperm cells to obtain a quantity of sperm cells having a desired characteristic, the quantity of sorted sperm cells being contained in a first volume of fluid having a first concentration of sperm cells; and subjecting the sperm cells to concentration steps in which the concentration of the sperm cells is increased to a second concentration greater than the first concentration.

Sperm cell processing methods

Staining mixtures comprising viable spermatozoa, a composition which regulates oxidation/reduction reactions intracellularly or extracellularly, and a DNA selective dye are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for staining sperm cells comprising the formation of a staining mixture are also disclosed.

Use of a composition which regulates oxidation/reduction reactions intracellularly and/or extracellulary in a staining or sorting process

Processes of storing sorted and unsorted spermatozoa, in the form of a sperm dispersion, having reduced motility relative to endogenous ejaculated sperm are disclosed. The immotile spermatozoa of the dispersion tend to have a greater capacity for enduring the rigors associated with storage, transportation, and fertilization procedures. Processes for providing such sperm dispersions, for inseminating a female mammal with such sperm dispersions, as well as compositions and combinations comprising the sperm dispersions, are also disclosed.

Process for storing a sperm dispersion

Sperm cell suspensions comprising a motility inhibitor are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for forming such cell suspensions, as well as processes for staining sperm cells, are also disclosed.

Sperm suspensions for sorting into X or Y chromosome-bearing enriched populations

Sperm cells are stained according to processes that involve the combining of sperm cells with a fluorescent DNA selective dye at an elevated temperature in excess of about 40&#xb0; C. The methods allow for a decreased staining time. The cells may thereafter be efficiently sorted according to common separation methods, including flow cytometry.

Process for the staining of sperm

The subject invention concerns methods and compounds that have utility in the treatment of a condition associated with cyclooxygenase-2 mediated disorders. Compounds of particular interest are benzopyrans and their analogs defined by formula 1 Wherein Z, X, R, R, R, and Rare as described in the specification.

Benzopyran compounds useful for treating inflammatory conditions

A class of benzopyrans, benzothiopyrans, dihydroquinolines, dihydronaphthalenes, and analogs thereof, is described for use in treating cyclooxygenase-2 mediated disorders. Compounds of particular interest are defined by Formula I' wherein X, A, A, A, A, R, R&#x2033;, Rand Rare as described in the specification.

Substituted benzopyran derivatives for the treatment of inflammation

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St. Louis, MO, United States of America

Cindy L Ludwig