Verily Life Sciences LLC

Insitro, Inc.

Ci Chu

Ian Peikon

Gary Tong

Daniel Liu

Jerrod Joseph Schwartz

Bi Yu Li

Max R Salick

Cynthia Hao

Charles Kim

Xiaomi Du

Provided herein are compositions and methods for the identification of an expression profile in a single cell or population of cells. Kits for use with the disclosed methods are also provided, including antibodies, with a unique molecular identifier and antibody identifier, and primers for amplification of the antibody identifier sequence.

Quantitative massively parallel proteomics

Disclosed herein are methods for performing in situ sequencing of RNA transcripts with non-uniform 5' ends. During reverse transcription (RT) of RNA transcripts, RT enzyme is induced to 'template-switch' to a separate oligonucleotide provided as the template for the upstream flanking region. This flanking region is grafted onto the beginning of the cDNA, enabling padlock probe detection, rolling circle amplification, and fluorescent in situ sequencing. Overall, the disclosed method for in situ sequencing can be applicable for analyzing exogenously introduced transcripts (e.g., identifying and determining impact of a perturbation including a CRISPR perturbation or shRNA/siRNA/ASO perturbation), analyzing naturally occurring transcripts (e.g., measuring gene expression, detecting splicing events), and analyzing modified, naturally occurring transcripts (e.g., detecting mutations or gene edits).

In situ sequencing of RNA transcripts with non-uniform 5 prime ends

Provided herein are methods for identification of an expression profile, a transcriptional profile, and/or an epigenetic profile from a cell-containing sample. Also provided are compositions for use in the disclosed methods.

Xseq

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Ci Chu