Chinese Academy of Agricultural Sciences

Chinese Academy of Sciences

Chunzhi Zhang

Sanwen Huang

Die Tang

Zhongmin Yang

Pei Wang

Ling Ma

Yanhui Zhu

Canhui Li

Yi Shang

Zhen Peng

Mingwang Ye

Provided is a StSCI protein for changing the self-incompatibility of diploid potato materials, wherein the amino acid sequence of the StSCI protein includes or consists of the following sequence: 1) the amino acid sequence represented by SEQ ID NO: 1; or 2) a functional homologous sequence having at least 95% sequence identity with the amino acid sequence represented by SEQ ID NO: 1; or 3) a protein in which one or more (e.g., 1-10) amino acids are added, deleted, or replaced in the amino acid sequence represented by SEQ ID NO: 1 and has the activity of inhibiting self-incompatibility. The advantage of the application is that the StSCI protein may inhibit the cytotoxicity of multiple types of S-RNase, which is hereditary and fundamentally overcomes the defect of self-incompatibility of diploid potatoes, thereby facilitating to realize the cultivation of a high-generation homozygous inbred line of diploid potatoes.

StSCI protein for changing self-incompatibility of diploid potato materials

Provided herein are materials and methods for producing diploid, fertile, uniform, and vigorous hybrid potato. Also provided are methods of using advanced breeding methods, such as genome design, to generate potato inbred lines with high homozygosity which enables the exploitation of heterosis in this tuber crop and transforms potato breeding from a slow, non-accumulative mode into a fast-iterative one.

Materials and methods for producing hybrid diploid potato

Disclosed is a method for breeding self-compatible potatoes, including the following steps: (1) selecting a self-compatible potato variety material and referring to it as PG6359, and cloning the S-RNase gene of PG6359 through the transcriptome sequencing method; and (2) obtaining two full-length sequences of the S-RNase gene from the cloned S-RNase gene in step (1) and referring to them as Sand Srespectively, and after carrying out an artificial self-pollination for the variety material PG6359, selecting the variety material having the genotype of SSfrom the offspring as the female parent, and selecting a self-incompatible material as the male parent, and then obtaining a self-compatible Fgeneration by hybridization. The invention overcomes the self-incompatibility of diploid potatoes, and does not require the introduction of any wild potato gene fragments, thereby avoiding linkage drag, and providing a basis for the rapid creation of a diploid potato inbred line.

Method for breeding self-compatible potatoes

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Shenzhen, China

Chunzhi Zhang