Zhongshan Guanghe Medical Technology Co., Ltd.

Fluorescence Diagnosis (shanghai) Biotech Company Ltd.

Chunyan Bao

Qiuning Lin

Linyong Zhu

Yang Liu

Xianjun Chen

Xuepeng Zhong

Bingkun Bao

Dasheng Zhang

Zengmin Du

Yujie Hua

Lipeng Yang

Yiqing Zhang

Yan Li

Yang Wayne Wang

Wei Fang

Renmei Liu

Yihui Ge

Zhenzhen Liu

Zhengda Chen

Sitong Zhang

Ningfeng Li

Xin Hua

This invention provides a preparation, composition, product, and application of a photo-coupled synergistically crosslinked hydrogel material. The preparation includes dissolving Component A including a photosensitive polymer derivative having o-nitrobenzyl phototriggers and Component B including a polymer derivative having amine or alkene (double group) or sulfhydryl group in a biocompatible medium to obtain solution A and solution B, respectively; mixing the solution A and solution B homogeneously to obtain a hydrogel precursor solution; initiating photo-coupled synergistic crosslinking under an irradiation of a UV light to form the hydrogel. The irradiation causes the o-nitrobenzyl phototriggers to generate an aldehyde group/keto group or a nitroso group to initiate photo-coupled synergetic crosslinking. The photo-coupled synergistically crosslinked hydrogel has applications in tissue engineering, regenerative medicine, 3D printing and as a carrier of cell, protein or drug.

Photo-coupled synergistically crosslinked hydrogel material and its composition, preparation method, use, product, and preparation kit

Provided are a fluorescent dye and a preparation process and use thereof. The fluorescent dye is able to emit a long wavelength, is sensitive and specific to viscosity, and is used for a wide range of purposes, such as viscosity testing, fluorescent labeling, quantification or detection of proteins, enzymes or nucleic acids. In addition, it can be used as a fluorescent-activated probe.

Fluorescent dye and preparation method and use thereof

Provided are a fluorescent probe and a preparation process and the use thereof. The fluorescent probe is sensitive and specific to viscosity, and can be used for specific fluorescent labeling of proteins, and can also be used for quantification, detection or kinetic studies of proteins, and the imaging of cells, tissues and living bodies.

Fluorescent probe and preparation method and use thereof

This invention provides preparations, compositions, products, and applications of photo-crosslinked hydrogels. Component A&#x2014;a photosensitive polymer derivative, component B&#x2014;the photoinitiator, and auxiliary component C&#x2014;other biocompatible polymer derivative each are respectively dissolved in a biocompatible medium to obtain solution A, solution B, and solution C. The solution A, the solution B, and the optional solution C are mixed homogenously to obtain a hydrogel precursor solution. The hydrogel precursor solution is subject to irradiation of the UV light for photocoupled crosslinking to form a photo-crosslinked hydrogel. The photo-crosslinked hydrogel exhibit rapid speed of photo-curing, strong tissue adhesion, excellent mechanical properties, good biocompatibility, and excellent clinical operability. In addition, this invention also provides a kit for making the photo-crosslinked hydrogel, and applications thereof in tissue engineering, regenerative medicine, 3D printing, and as a carrier of cell, protein, or drug.

Photo-crosslinked hydrogel material and preparation, composition, and application thereof photo-crosslinked hydrogel

A method for preparing non-free radical photo-crosslinked hydrogels includes: dissolving component A that is a polymer derivative modified with o-nitrobenzyl phototrigger in a biocompatible medium to obtain solution A; dissolving component B that is a polymer derivative containing hydrazide, hydroxylamine or primary amine in a biocompatible medium to obtain solution B; mixing solution A and solution B to obtain a precursor solution of hydrogel; under light irradiation, crosslinking aldehyde generated from the o-nitrobenzyl with the hydrazine, hydroxylamine or primary amine to obtain a hydrogel by forming hydrazone, oxime or schiff base, respectively. A kit for preparation and application of the hydrogel in tissue repair, beauty therapy, and cells, proteins or drugs carriers is also described. The method or kit can achieve in situ photo-gelling on tissue surface or in situ forming thin gel on wounds in clinical treatment of wounds.

Preparation method, product and application of non-free radical photo-crosslinked hydrogel material

Growing community of inventors

Shanghai, China

Chunyan Bao