Other

Jiangnan University

Bright Dairy & Food Co., Ltd.

Jiangsu Yunzhong Medical Technology Co., Ltd.

Chunmei Chen

Jian Chen

Guocheng Du

Jianghua Li

Long Liu

Xueqin Lv

Jieying Deng

Kai Zhu

Yong Feng

Jianxin Xue

Yun Guo

Xiao Li

Zengjun Wang

The present disclosure provides a stapler, including a shell, an ejector plate pin, a cartridge pin, an anvil pin, an unlocking pin, an ejector plate ring, a cartridge ring and an anvil ring, where the cartridge pin is between the ejector plate pin and the anvil pin; the ejector plate ring, the cartridge ring and the anvil ring all include a first half ring and a second half ring; hinged ends of the first half rings and the second half rings are rotatably connected, and fixed ends of the second half rings of the ejector plate ring, the cartridge ring and the anvil ring are connected to the front ends of the ejector plate pin, the cartridge pin and the anvil pin, respectively. The present disclosure adopts a brand-new concept of eversion anastomosis, and ensures the smoothness of a lumen after anastomosis.

Stapler

The disclosure discloses recombinantfor synthesizing guanosine diphosphate fucose and a construction method and application thereof. The recombinantis obtained by intensively expressing guanylate kinase and nucleotide diphosphokinase genes and expressing exogenous fucokinase and phosphate guanylyltransferase genes in a genome of168. According to the disclosure, a bacterial strain for synthesizing the guanosine diphosphate fucose is obtained by reconstructing the168, with a volume of intracellular accumulation up to 196.15 g/L. According to the disclosure, by intensively expressing the guanylate kinase and nucleotide diphosphokinase genes, and enhancing the supply of intracellular GDP-L-fucose composition cofactors, the synthesis of the guanosine diphosphate fucose is promoted. The construction method for the recombinantof the disclosure is simple and convenient to use, thus having good application prospects.

Recombinantfor synthesizing GDP-L-fucose and application thereof

Growing community of inventors

Wuxi, China

Chunmei Chen