Shenzhen Mindray Bio-medical Electronics Co., Ltd.

Shenzhen Yhlo Biotech Co., Ltd.

Chungen Qian

Yuping Zhang

Kunhui Hu

Gang Wang

Ke Li

Fuzhen Xia

Dingbiao Zou

Liang Zhu

Mi Zhou

Yujian Lin

Liang Zheng

Xiaoqin Ye

Zhisheng Dang

Xiaotao Lin

Fangming Cheng

Liqiang Wu

Chunlei Luo

Chengyong Xiao

Taoxu Liu

Meiting Liu

The disclosure relates to the technical field of virus detection, in particular, to an IgA antibody and a kit capable of specifically recognizing RBD protein. The antibody can be used as a calibrator of IgA antibody against the RBD protein of SARS-CoV-2. the disclosure further relates to a kit comprising the antibody which enables automated, high-throughput, and rapid detection of IgA antibody against novel coronavirus pneumonia.

IgA antibodies and kits capable of specifically recognizing RBD protein

Provided are a leptin immunogen, hybridoma cells, a monoclonal antibody, a polyclonal antibody and use thereof. The leptin immunogen includes a polypeptide having an amino acid sequence as shown in SEQ ID NO:. The anti-leptin monoclonal antibody prepared with the leptin immunogen has good specificity and high sensitivity.

Leptin immunogens, hybridoma cells, monoclonal antibodies, polyclonal antibodies and use thereof

Provided in the present invention are a magnetic nanosphere coated with modified cardiolipin, and manufacturing method thereof. The magnetic nanosphere coated with modified cardiolipin comprises a modified cardiolipin, a biotin derivative, and a streptavidin magnetic bead. The modified cardiolipin is coupled to the biotin derivative via an &#x2014;NH&#x2014;CO structure. The streptavidin magnetic bead is a magnetic nanosphere coupled to streptavidin, and the biotin derivative is coupled to the streptavidin.

Modified cardiolipin-coated magnetic nanobeads and preparation methods therefor

Disclosed a chemiluminescence enhancer and a chemiluminescence immunodetection kit. The chemiluminescence enhancer is specifically a betaine and can be used for enhancing the luminance of a luminescent acridine substance. The chemiluminescence immunodetection kit comprising the chemiluminescence enhancer and a luminescent acridine substance features greatly enhanced luminescence signals and high detection sensibility, and can be widely applied to fields such as clinical diagnoses, scientific research, and sanitation analysis.

Chemiluminescence enhancer and chemiluminescence immunodetection kit

An enhancing reagent for enhancing chemiluminescence of 1,2-dioxetane compounds and a method for using the enhancing reagent to enhance the chemiluminescence are provided, in which the enhancing reagent contains an alkyl bis-quaternary ammonium salt of Formula I. A chemiluminescent composition with a 1,2-dioxetane compound as a substrate and a kit thereof are further provided, which contain a 1,2-dioxetane compound and an alkyl bis-quaternary ammonium salt of Formula I. 

Chemiluminescent compositions and enhancing reagents for chemiluminescence

Methods for synthesizing an ALP conjugate are provided. The methods may include activating a carboxyl group of the ALP with a carbodiimide, to generate an active ester; and adding the substance to be conjugated such that a synthetic reaction occurs between the active ester and the substance to be conjugated, to generate an ALP conjugate.

Processes for synthesizing alkaline phosphatase conjugates

A enhancing reagent for enhancing chemiluminescence of 1,2-dioxetane compounds and a method for using the enhancing reagent to enhance chemiluminescence are provided, in which the enhancing reagent contains a multi-alkyl quaternary ammonium salt of Formula I. A chemiluminescent composition with a 1,2-dioxetane compound as a substrate and a kit thereof are further provided, which contain a 1,2-dioxetane compound and a multi-alkyl quaternary ammonium salt of Formula I. 

Chemiluminescent compositions, enhancing reagents for chemiluminescence and methods for the preparation and use thereof

The present disclosure relates to stabilizers for alkaline phosphatase or conjugates thereof, a process for preparing a stabilizer, and a method for stabilizing alkaline phosphatase or conjugates thereof with a stabilizer. The present disclosure also relates to a reagent of alkaline phosphatase or conjugates thereof as well as to a process for preparing the same. In another aspect, the present disclosure relates to a kit comprising the stabilizers disclosed herein and alkaline phosphatase or conjugates thereof. The stabilizer disclosed herein can stabilize alkaline phosphatase or conjugates thereof for a prolonged period of time, extending their shelf-life.

Reagents and processes for stabilizing alkaline phosphatase or conjugates thereof

A enhancing reagent for enhancing chemiluminescence of 1,2-dioxetane compounds and a method for using the enhancing reagent to enhance the chemiluminescence are provided, in which the enhancing reagent contains an alkyl bis-quaternary ammonium salt of Formula I. A chemiluminescent composition with a 1,2-dioxetane compound as a substrate and a kit thereof are further provided, which contain a 1,2-dioxetane compound and an alkyl bis-quaternary ammonium salt of Formula I. 

The present disclosure provides a chemiluminescent composition where the chemiluminescent substrate is 1,2-dioxetane, which contains 1,2-dioxetane and an alkyl dimethyl benzyl quaternary ammonium salt having the structure of general formula I. The present disclosure also provides an enhancing reagent and methods for enhancing chemiluminescence of 1,2-dioxetane, where the enhancing reagent contains an alkyl dimethyl benzyl quaternary ammonium salt having the structure of general formula I. 

Methods for preparing conjugates including enzyme conjugates and especially alkaline phosphatase (ALP) conjugates, and a kit are provided. The methods include: blocking an amino group on a molecule surface of a first substance to be conjugated containing an amino group and a carboxyl group (for example, an enzyme) with a carboxyl compound; adding a carbodiimide to activate the first substance to be conjugated with the amino group blocked; inactivating or removing the carbodiimide; and adding a second substance containing an amino group (for example, a substance to be labeled). Conjugates (for example, enzyme conjugates) are obtained.

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Shenzhen, China

Chungen Qian