Academia Sinica

Other

Academic Sinica

Chung-Hsuan Chen

Jung-Lee Lin

Ming-Lee Chu

Yi-Sheng Wang

Michael Hsiao

Chen-Yu Hsieh

Nien-Yeen Hsu

Olga K Kosheleva

Peter Lai

Nelson G Chen

Alice L Yu

Chin-Yu Wang

Hsin-Hung Lin

Huan-Cheng Chang

Hsin Yu Hsieh

Wen-Ping Peng

Shang-Ting Tsai

Huan-Chang Lin

Chen-Ren Hsiao

Chiu Wen Chen

Cai-Yu Kao

Abdil Ozdemir

I-Chung Lu

Wei-Chao Chang

Ming-Shyan Huang

Bai-Ling Lin

Chih-Jen Yang

Kent J Gillig

Huan Chang Lin

Chein-Hung Chen

Chien-Hsun Chen

Pang-Hung Hsu

Chen-I Wu

Apparatus and methods for high performance liquid chromatography. The apparatus includes a preparation loop comprising two linear stepping pumps, a sample loop comprising a sample injector, a chromatography column, and a detector device. The detector device can include a flash lamp, a flow cell, and a light sensor comprising an entrance slit, a grating; and a charge-coupled device array.

High performance liquid chromatography with UV-visible detection

Disclosed herein is a device for characterizing a biological sample or an airborne sample. According to embodiments of the present disclosure, the device comprises an electrospray source, a mass analyzer, a charge detector, and optionally, an ion guide. The present device is useful in analyzing the particle population in the biological or airborne sample based on the mass to charge (m/z) ratio and the charge (z) of each particle. Also disclosed herein are the methods of making a diagnosis of cancer by use of the present device, and methods of determining the mass distribution of particles in an airborne sample.

Device for characterizing particles and uses thereof

An ion trap mass spectrometer and methods for obtaining a mass spectrum of ions by scanning an RF frequency applied to the linear ion trap for mass selective ejection of the ions by using two power amplifiers to apply opposite phases of the RF to x and y electrodes.

Frequency scan linear ion trap mass spectrometry

Disclosed herein is an aptamer targeting a MHC-presented peptide and its uses thereof. The MHC-presented peptide is expressed in various cancer cells; therefor, the aptamer of the disclosure is useful as a bio-tool to label and/or treat peptide-presenting cancer cells. Also disclosed herein is a pharmaceutical composition containing the aptamer.

Aptamer targeting MAGE-A3 peptide and uses thereof

Apparatus and methods for creating a pulsed ion beam. The pulsed ion beam can be used for performing mass spectrometry. A pulsed solenoid valve can provide a pulsed ion beam from an electrospray in a pre-vacuum chamber. The pulsed ion beam can enter a high vacuum region and a mass analyzer for mass spectrometry.

Pulsed ion beam source for electrospray mass spectrometry

Apparatus and methods for automatic amino acid sequencing of a glycopeptide by mass spectrometry. The glycopeptide is fragmented by higher energy collision dissociation fragmentation, and sequentially fragmented by collision induced dissociation fragmentation. The glycopeptide Y1 ion is isolated, and the mass spectra of fragmented glycopeptide Y1 ions provide mass spectral peaks corresponding to the amino acid sequence of the glycopeptide.

Automatic amino acid sequencing of glycopeptide by Y1 ions

Methods for determining the quality of a biomolecular microarray to determine suitability of the microarray for performing specific binding reactions, such as hybridization, are provided. Methods are based on staining a microarray with a solution of detectable nanoparticles that reversibly stain the biomolecules through an electrostatic interaction to select microarrays that meet quality standards for hybridization reactions. A gold nanoparticle solution based staining method for DNA microarrays is provided. Destaining methods allowing multiple rounds of hybridization of nanogold stained microarrays are provided. Microarrays selected by methods of the invention are provided.

Characterization of microarrays by nanogold staining

Methods for detecting the presence of nanoparticles or microparticles by cell mass spectrometry (CMS) are provided. CMS methods are provided for determining the number of nanoparticles or microparticles in each cell. Nanoparticles whose intracellular concentration can be determines by the CMS methods of the invention include polymeric nanoparticles (NPs), liposomes, viral-based NPs, carbon nanotubes, diamond NPs, polymeric micelles, nanocarriers, liposomes, and viral nanoparticles. Determination of the efficiency of drug delivery and intracellular titer of pathogens according to the invention is disclosed. Methods for determining intracellular uptake of virus particles are provided.

Quantitative measurement of nano/micro particle endocytosis with cell mass spectrometry

Methods for reducing the growth of breast cancer cells in a subject by exposing the cells to nanoparticles, and irradiating with a focused, low to medium power ultrasound. The nanoparticles can be gold or magnetic nanoparticles. The nanoparticles can have a cancer drug attached, such as an antibody-based cancer drug.

Nanoparticle-assisted ultrasound for breast cancer therapy

Methods for diagnosis of non-small cell lung cancers by detection of endogenous peptides in exhaled breath condensate (EBC) are provided. Diagnostic peptides derived from dermcidin (DC) are provided. A specific dermcidin-derived peptide E-R11, having the sequence ENAGEDPGLAR (SEQ ID NO:2), is provided. E-R11 peptide levels in EBC, as measured by mass spectrometry (MS), are highly diagnostic of non-small cell lung cancers. A method for inhibiting growth of lung cancer cells by inhibiting DCD expression by RNA interference also is provided.

Dermcidin-derived peptides for lung cancer diagnostics

A periodic field differential mobility analyzer apparatus for separating and identifying ionic analytes employs a series of elongated parallel channels, a pump, a first voltage providing an electric field Ein a direction opposing the gas flow, a second voltage providing an electric field Ein a direction perpendicular to the gas flow, an ion source, and a detector. The periodic field differential mobility analyzer provides high resolution and sensitivity.

Periodic field differential mobility analyzer

Methods and apparatuses for portable mass spectrometry are disclosed. The apparatuses comprise at least one source of ionized analyte, at least one frequency scanning subsystem, at least one detector, and optionally at least one vacuum pump, and are portable. In some embodiments, the apparatuses comprise multiple sources of ionized analyte and/or are configured to obtain mass spectra of a large analyte, such as analyte with an m/z ratio of at least 10, or analyte with a molecular weight of at least 10Da, as well as mass spectra of small molecule analyte. In some embodiments, the methods comprise obtaining mass spectra with a portable apparatus described above.

Apparatuses and methods for portable mass spectrometry

Methods for killing cancer cells and treating cancer in a subject by exposing the cells to nanoparticles, and irradiating with a focused, low to medium power ultrasound. The nanoparticles can be gold, iron oxide, copper, silver, polystyrene, PEG, or liposome nanoparticles. The nanoparticles can have a cancer drug attached, such as an antibody-based cancer drug.

Nanoparticle-assisted ultrasound for cancer therapy

Apparatus and methods for performing mass spectrometry of a nanoparticle or virus analyte. Apparatus may include a laser desorption plate, a mass analyzer configured to measure mass over the range of m/z from 10to 10, an electrical shield surrounding the mass analyzer, and a charge sensitive detector, wherein the laser firing is phase lock synchronized with the applied radiofrequency voltages.

Growing community of inventors

Taipei, Taiwan

Chung-Hsuan Chen