National Institute of Advanced Industrial Science and Technology

Chun Wu

Yoshihiro Ohmiya

Satoru Ohgiya

Kosei Kawasaki

The present invention provides a method for detecting the presence or absence of a guanine-abasic site, the method being a process for detecting guanine opposite at least one abasic sites generated in a double-stranded DNA, comprising:

Method for detecting guanine-abasic site in DNA

The present invention relates to a method for producingluciferase labeled with hydrophilic biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted withluciferase, and biotin-labeledluciferase wherein a sugar chain inluciferase has been biotinylated.

[object Object]

The present invention is one gene construct or a combination of two gene constructs or expression vectors incorporating aluciferase gene and a copepod luciferase under the control of distinct promoters. These gene constructs and expression vectors are useful for making a mammalian cell incorporating theluciferase gene and the copepod luciferase to be capable of stably expressed and extracellularly secreted under the control of the distinct promoters.

Two secretory luciferases

The present invention relates to a method for producing marine ostracod crustacean luciferin or a derivative thereof represented by a general formula (4), characterized by reacting a compound represented by a general formula (2) with a compound represented by a general formula (3): wherein R, R, R, R, Yand Zare the same as defined in the specification.

Luciferin luminescent substrate of marine ostracod crustacean and method for production thereof

An object of the present invention is to produce a luminescent probe that has less biological effects, efficiently emits visible to near-infrared light, which is excellent for the imaging of individuals, and the use thereof. The present invention provides a sugar chain-containing-luciferase derivative, wherein an organic fluorescent dye is bonded to the luciferase through the sugar chain.

Visible to near-infrared light probe using energy transfer between luciferase and an organic dye via a sugar chain

Method for producing substituted imidazo[1,2-A]pyrazines of (s)-1-(3-(2-sec-butyl-6-(1h-indol-3-yl)-3-oxo-3,7-dihydroimadazo[1,2-a]pyrazin-8-yl)propyl)guanidine

The present invention relates to a stabilization composition of coelenterazine or an analog thereof, a kit, a method for measuring a coelenterazine-based biological luminescence, containing coelenterazine or the analog thereof and an antioxidant.

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Ikeda, Japan

Chun Wu