Takara Bio USA, Inc.

Chun-Wah Lin

Jude Menlo Dunne

Maithreyan Srinivasan

Thomas David Schaal

Alain-Albert Mir

Shanavaz Loharasp D Nasarabadi

Syed A Husain

Patricio A Espinoza Vallejos

Patricio Espinoza

Hermann Hubschle

Patricio A Espinoza-Vallejos

Provided are methods, devices, assemblies, and systems for dispensing into the wells of a multi-well device and imaging such wells from multiple Z-planes. Multi-Z imaging of the present methods and systems may allow for the detection of wells of a multi-well device that contain a desired number of cells. Also provided are methods, devices, assemblies, and systems for processing cell-containing wells of a multi-well device identified through the use of multi-Z imaging.

Multi-Z imaging and dispensing with multi-well devices

The present disclosure provides methods, device, assemblies, and systems for dispensing and visualizing single cells. For example, provided herein are systems and methods for dispensing a dispense volume into a plurality of wells of a multi-well device, where, on average, a pre-determined number of cells (e.g., 1-20) are present in the dispense volume, and determining, via a cellular label, the number of cells present in each of the plurality of wells. Such dispensing and cell detection may be repeated a number of times with respect to wells identified as having less than the pre-determined number of cells in order increase the number wells in the multi-well device containing the desired number (e.g., a single cell).

Method for rapid accurate dispensing, visualization and analysis of single cells

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Fremont, CA, United States of America

Chun-Wah Lin