Korea Research Institute of Bioscience and Biotechnology

Chun Suk Kim

Jung Oh Ahn

Eun Gyo Lee

Hyeok Won Lee

Hong Weon Lee

Joon Ki Jung

Eui Sung Choi

Myoung Soo Park

Ji Yeon Hong

Disclosed herein are a-derived auto-inducible NPS promoter; a recombinant expression vector carrying the promoter and a nucleotide sequence, the sequence being operably linked to the promoter and coding for a recombinant protein; a host cell transformed or transfected with the recombinant expression vector; and a method for producing a recombinant protein, comprising culturing the host cell to express the recombinant protein and isolating the protein. The NPS promoter allows a recombinant protein of interest to be produced on a large scale without the need of any inducer.

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Disclosed are a-derived translation elongation factor (TEF) promoter, a recombinant expression vector carrying the promoter and a heterologous protein encoding base sequence operably linked to the promoter, and a host cell transfected or transformed with the recombinant expression vector. Also, a method is provided for producing a heterologous protein. The method comprises culturing the host cell to express the-derived TEF promoter and isolating the promoter from the culture. The TEF promoter can be utilized for the mass production of heterologous proteins without the need for inducers.

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Daejeon, South Korea

Chun Suk Kim