Yonsei University

Institute for Basic Science

Chul Yong Park

Dong Wook Kim

Jin Soo Kim

Jung Eun Kim

Jiyeon Kweon

Duk Hyoung Kim

Ji Yeon Kweon

The present invention provides a method for inducing an inversion of normal blood coagulation factor VIII (F8) gene, a method for correcting an inversion of blood coagulation factor VIII gene in which the inversion has occurred, and a Hemophilia A patient-derived induced pluripotent stem cell in which the inversion is corrected, constructed using the same. The method of the present invention effectively reproduces the inversion of intron 1 and intron 22 of the F8 gene, which is responsible for the majority of severe hemophilia A, and thereby may be effectively used for studying the development mechanism of hemophilia A and as a research tool for screening therapeutic agents. The inversion-corrected induced pluripotent stem cell constructed according the method of the present invention enables an efficient and fundamental treatment for hemophilia A by restoring a genotype in which mutation has occurred to a wild type-like state, without limitation via normal gene or protein delivery.

RNA guided endonuclease targeting blood coagulation factor VIII intron 1 inversion gene and composition for treating hemophilia comprising same

Talen targeting blood coagulation factor VIII intron 1 inversion gene and composition for treating hemophilia comprising same

Endonuclease targeting blood coagulation factor VIII gene and composition for treating hemophilia comprising same

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Seoul, South Korea

Chul Yong Park