National University of Singapore

Chui Yee Fong

Tuan Ariffeen Bongso

Mahesh Choolani

Arijit Biswas

Seeram Ramakrishna

Ariffeen Bongso

Sukumar Ponnusamy

Daniel Hao Lin

Zhouwei Huang

Gauthaman Kalamegam

The invention is directed to a method of treating a wound (e.g., to suppress scar formation) in an individual in need thereof comprising contacting the wound with an effective amount of a composition comprising (i) Wharton's jelly stem cells (WJSCs), (ii) a cell culture medium that has been conditioned with WJSCs, (iii) a lysate of WJSCs, (iv) a cell culture medium that has been conditioned with WJSCs exposed to apoptotic skin cells and keloid cells, or (v) a combination thereof. The invention is directed to a medical dressing (e.g., pharmaceutical compositions) comprising Wharton's jelly stem cells (WJSCs), a cell culture medium that has been conditioned with WJSCs, a lysate of WJSCs, a cell culture medium that has been conditioned with WJSCs exposed to apoptotic skin cells and keloid cells, or a combination thereof.

Wound dressing nanomesh impregnated with human umbilical cord Wharton's jelly stem cells

There is provided a method of identifying at least one foetal erythroblast in a sample, the method comprising analyzing the morphology of at least one cell in the sample; wherein at least one analyzed cell that is nucleated, is CD45 negative and comprises a relatively high cytoplasmic to nuclear ratio is identified as the foetal erythroblast.

Method of identifying, isolating and/or culturing foetal erythroblasts

The invention is directed to a method of freezing stem cells comprising introducing the HSCs into a cell culture medium that has been conditioned with Wharton's Jelly mesenchymal stem cells (WJSCs), thereby producing a stem cell culture, and slowly freezing the stem cell culture. In other aspects, the invention is directed to compositions comprising stem cells produced by the methods provided herein. In yet other aspects, the invention is directed to pharmaceutical compositions comprising the stem cells produced by the methods provided herein.

Methods of freezing stem cells

In one aspect, the invention is directed to methods of expanding hematopoietic stem cells (HSCs) comprising culturing the HSCs with Wharton's Jelly mesenchymal stem cells (WJSCs), a cell culture medium that has been conditioned with WJSCs, or a combination thereof, thereby producing a HSC culture; and maintaining the HSC culture under conditions in which the HSCs expand in the culture, thereby expanding the HSCs. In another aspect, the invention is directed to a method of transplanting the expanded HSCs in an individual in need thereof. In yet another aspect, the invention is directed to compositions comprising HSCs and Wharton's Jelly mesenchymal stem cells (WJSCs). The composition can further comprise a cell culture medium that has been conditioned with WJSCs.

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Singapore, Singapore

Chui Yee Fong